摘要
目的:观察葡萄内酯对氢溴酸东莨菪碱致大鼠学习记忆障碍的影响,并初步探讨其作用机制。方法:将大鼠随机分为空白组、模型组、阳性药物组以及葡萄内酯低、中、高剂量组,除空白组采用腹腔注射(ip)生理盐水外,其余各组均采用ip东莨菪碱造模,葡萄内酯低、中、高剂量组分别按0.514,0.771,1.028 mg·kg-1灌胃(ig)给药,阳性药物组给予多奈哌齐0.514 mg·kg-1,连续14 d。用Morris水迷宫测定大鼠空间学习记忆能力;并采用紫外分光光度法检测大鼠脑组织中超氧化物歧化酶(SOD),乙酰胆碱酯酶(AChE)的活性以及丙二醛(MDA)和谷氨酸含量。结果:葡萄内酯可以缩短大鼠的逃避潜伏期(P<0.01),葡萄内酯各剂量组可降低AChE活性(P<0.05),其中,葡萄内酯中、高剂量组与模型组比较差异有极显著性(P<0.01);葡萄内酯高剂量组均可降低脑组织中谷氨酸含量(P<0.01);而各剂量组葡萄内酯对SOD活力的提高和MDA含量降低无明显影响。结论:葡萄内酯可以提高对东莨菪碱致学习记忆障碍大鼠的学习记忆能力,其机制可能与和降低AChE活性有关。
OBJECTIVE To investigate the rats, and its related mechanism. METHODS effects of auraptene on learning and memory deficits induced by scopolamine in SD rats were randomly divided into six groups, control group, model group , Aricept-treated group （0. 514 mg·kg^-1, ig）, and auraptene-treated group （0. 514, 0. 771, 1. 028 mg·kg^-1, ig）. Except the control group were intraperitoneally injected with isotonic saline, the rest of rats were intraperitoneally injected with scopolamine for 14 days and administered with the related medicines. The spatial learning performance was evaluated by Morris water maze experiment. The activity of superoxide dismutase （SOD）, acetylcholinesterase （ACHE）, and the content of malondialdehyde （MDA） and glutamic acid were assayed with ultraviolet spectrophotometry. RESULTS Auraptene （0. 514 mg·kg^-1 -1. 028mg·kg^-1） could decrease the escape latency of rats （P〈0. 01）. Auraptene could decrease the activity of AChE （P〈0. 05）, espe cially auraptene （0. 771, 1. 028 mg·kg^-1 ） groups could more significantly decrease the activity of AChE （P〈0. 01）. Auraptene （1. 028 mg·kg^-1 ） could significantly decrease content of giutamic acid （P〈0.01）. Auraptene had no effect on the activity of SOD and the content of MDA. CONCLUSION Auraptene could improve the learning and memory deficits induced by scopolamine in rats,and its mechanism may be related to the decrease in the activity of ACHE.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2014年第3期167-170,共4页
Chinese Journal of Hospital Pharmacy
基金
科技部"十一五"新药创制重大专项(编号:2009ZX09103-350)
国家自然科学基金资助项目(编号:81260612)
江西省自然科学基金(编号:20114BAB205072)
作者简介
[作者简介]陈海芳，女，讲师，研究方向：药效物质基础和药效研究，E-mail：chenhf88@126．com
[通讯作者]杨武亮，男，教授，研究方向：药效物质基础和药效研究及质量标准研究，电话：0791-7118659，E-mail：yangwuliang@163．com
