摘要
目的 给予大鼠饲喂高脂饮食和灌注白酒分别制造大鼠高脂饮食性和酒精性脂肪肝模型 ,观察两种模型肝脏形态学和超微结构的区别。方法 选用 4～ 6周龄雄性SD大鼠 45只 ,分为 3组 :正常对照组 ,只给予普通饲料 ;高脂饮食组 ,用高脂饲料喂养 ,连续 8周 ;酒精组 ,用体积分数为 6 0 %白酒灌胃 ,初始剂量为 8g/kg ,每 2周增加 2g ,直到 16 g/kg维持 16周。试验末 ,将动物麻醉 ,取部分肝组织制备病理切片 ,另取部分制备电镜。结果 正常对照组大鼠肝组织形态结构正常 ,肝小叶规则 ,无脂滴发现 ;高脂饮食组可见多处脂质沉积 ,但尚无明显的肝纤维化表现 ,电镜下可见肝细胞胞浆内有大量脂肪滴积聚 ;酒精组脂肪肝大鼠肝细胞有脂肪变性的表现 ,电镜下可见类结晶体 ,线粒体肿胀 ,内质网扩张、断裂 ,数量较少 ,溶酶体内有电子密度增高的颗粒沉积 ,有一定量的脂滴。结论 大鼠高脂饮食性脂肪肝超微结构改变主要以肝细胞细胞浆内脂肪滴沉积为主 。
Objective To evaluate morphologic and ultrastructural differences between fatty liver due to high fat diet and alcoholic fatty liver due to spirits. Methods Forty five male SD rats aged 4～6 weeks were divided into 3 groups:normal control group fed common feed; high fat diet group, continuously fed high fat diet for 8 weeks;alcoholic group: fed 60% spirits with initial dose of 8 g/kg,and doses were increased by 2 g/kg every 2 weeks until the dose reached 16 g/kg and then maintained for 16 weeks.At the end of the experiments,the animals were anesthetized and liver tissues were obtained.One part of liver was used as pathologic sections and the other part was for electron microscopy. Results In normal group,hepatic lobules were well arranged and there were no lipid droplets; in high fat diet group,there were a lot of lipid deposition and generous fat accumulation was also observed by electron microscopy;in alcoholic fatty liver group,fatty degeneration of hepatocyte was observed. Similar crystal,swelling of mitochondrion,dilatation and disruption of endoplasmic reticulum as well as particles deposition of high electronic density and lipid droplet also were found by electron microscopy. Conclusion Main ultrastructural changes of fatty liver in rats fed high fat diet were lipid deposition in hepatocyte,though in alcoholic fatty liver,main changes were mitochondrion swelling and degeneration and structural alteration of other organellae.
出处
《山西医科大学学报》
CAS
2001年第1期1-3,共3页
Journal of Shanxi Medical University
