摘要
为探明缺磷胁迫下草甘膦对抗草甘膦大豆幼苗叶片光合作用和叶绿素荧光参数的影响,采用溶液培养方法,在大豆长出真叶时进行缺磷胁迫,第二复叶完全展开时进行草甘膦处理,5d后测定各生理指标。结果表明:相对于正常供磷条件的清水处理,缺磷胁迫下4.98 mL/L草甘膦处理的大豆叶片净光合速率(Pn)、气孔导度(Gs)、胞间CO2浓度(Ci)、最大荧光(F m)、PSⅡ最大光化学效率(F v/F m)、PSⅡ的有效量子产量[Y(Ⅱ)]、PSⅡ非调节性能量耗散的量子产量[Y(NO)]、最大电子传递速率(ETR max)和半饱和光强(I k)均呈下降趋势。而气孔限制值(Ls)、叶绿素a(Chl a)、叶绿素b(Chl b)、叶绿素a/b(Chl a/b)、类胡萝卜素(Car)、总叶绿素(Chl)含量和PSⅡ调节性能量耗散的量子产量[Y(NPQ)]均呈升高趋势。说明缺磷胁迫和喷施草甘膦显著降低了抗草甘膦大豆的光合速率。缺磷引起的气孔因素可能是导致大豆光合速率下降的主要原因,而光合速率的下降导致其PSⅡ反应中心的开放程度降低,活性减弱,参与CO2固定的电子较少,光化学效率较低。
Effect of glyphosate on photosynthesis and chlorophyll fluorescence parameters of leaves of glyphosate-resistant soybean seedlings （RR1） under phosphorus deficiency stress was studied using a solution culture experiment under phosphorus deficiency stress at the true leaf stage. The glyphosate at 4.98 mL/L was sprayed at the two-trifoliolate leaf stage, and physiological indexes were analyzed 5 days after the glyphosate spraying. The results show that under the phosphorus deficiency stress, the photosynthetic rate （Pn）, stomatal conductance （Gs）, intercellular CO2 concentration （Ci）, maximum fluorescence （Fm）, PSII maximum quantum yield （Fv/Fm）, PSII effective quantum yield[Y（Ⅱ）], PS II energy dissipation in non-regulatory quantum yield[Y（NO）], half saturation light intensity （IK） and maximum electron transport rate （ETRmax） in leaves of RR1 treated by 4.98 mL/L glyphosate are lower than those treated by water under normal phosphorus condition, while the stomatal limitation value （Ls）, chlorophyll a （Chl a）, chlorophyll b （Chl b）, chlorophyll a/b （Chl a/b）, carotenoid （Car）, total chlorophyll （Chl） content and regulation of PSⅡ quantum yield of energy dissipation[Y（NPQ）] treated by glyphosate under phosphorus deficiency stress are higher than those treated by water under normal phosphorus conditions. It suggests that spraying herbicide glyphosate under phosphorus deficiency stress decreases photosynthetic rate in leaves of Roundup Ready Soybean significantly. The stomatal limitation resulted from phosphorus deficiency stress may be the main reason for the decline of photosynthetic rate, and the decreased photosynthetic rate leads to reduce the openness of PSⅡ reaction center, PSⅡ activity, electrons in CO2 fixation and photochemical efficiency.
出处
《植物营养与肥料学报》
CAS
CSCD
北大核心
2014年第1期221-228,共8页
Journal of Plant Nutrition and Fertilizers
基金
国家引进国际先进农业科学技术"948"计划(610022)
山西农业大学引进人才科研启动项目(XB2009018)资助
关键词
缺磷胁迫
草甘膦
抗草甘膦大豆
光合作用
叶绿素荧光参数
phosphorus deficiency stress
glyphosate
glyphosate-resistant soybean
photosynthesis
chlorophyllfluorescence parameter
作者简介
原向阳（1981-），男，山西省阳城县人，副教授，博士，主要从事作物化学调控与化学除草方面的研究。Tel：0354-6289272，E—mail：yuanxiangyang200@163．com
通信作者Tel：0354-6286938，pyguo@$xau．edu．cn
