摘要
目的：观察肺气肿合并间歇低氧暴露对大鼠心肌细胞氧化应激损伤的作用。方法60只雄性Wistar大鼠按随机数字表法分为4组，每组15只。正常组正常饲养；肺气肿模型组每日常氧下熏烟2次，每次30 min；间歇低氧对照组每日间歇低氧暴露8 h；肺气肿合并间歇低氧干预组每日常氧熏烟2次，每次30 min，并间歇低氧暴露8h；各组均暴露8周。于第9周初每组随机取5只大鼠测定血气；剩余大鼠处死，取肺组织，苏木素-伊红（HE）染色、光镜下观察肺组织病理形态的变化；肺组织病理形态和血气分析结果证明，肺气肿合并间歇低氧大鼠模型建立成功。取心脏组织，采用酶联免疫吸附试验（ELISA）测定丙二醛（MDA）和超氧化物歧化酶（SOD）水平，实时逆转录-聚合酶链反应（RT-PCR）测定还原型辅酶Ⅱ（NADPH）氧化酶p22phox亚基mRNA表达水平。结果与正常组比较，肺气肿模型组、间歇低氧对照组和肺气肿合并间歇低氧干预组心肌MDA含量及p22phox mRNA表达水平均明显升高〔MDA（μmol/g）：2.93＆#177;0.54、3.58＆#177;0.63、4.51＆#177;0.72比1.75＆#177;0.56，p22phox mRNA：0.043＆#177;0.004、0.067＆#177;0.015、0.123＆#177;0.016比0.018＆#177;0.002，均P＜0.05〕，SOD水平均明显降低（U/mg：36.07＆#177;4.79、33.51＆#177;7.12、24.29＆#177;5.36比46.08＆#177;5.12，均P＜0.05）；其中肺气肿合并间歇低氧干预组MDA、p22phox mRNA表达升高及SOD降低水平较肺气肿模型组、间歇低氧对照组更明显（均P＜0.05）。p22phox mRNA表达水平与MDA含量呈显著正相关（r＝0.734，P＜0.001）。结论肺气肿合并间歇低氧暴露相对于单纯肺气肿或间歇低氧暴露对大鼠心肌组织能造成更严重的氧化应激损伤，NADPH氧化酶可能是其氧化应激反应的重要介质。
Objective To observe the effect of exposure of emphysema with intermittent hypoxia on oxidative stress injury of myocardial cells in rats. Methods Sixty male Wistar rats were divided randomly into four experimental groups（each n=15）. The normal control group was bred normally. The emphysema group was exposed to cigarette smoke twice a day（once 30 minutes）. The intermittent hypoxia（IH）group was exposed to intermittent hypoxia circumstance 8 hours/day,and the emphysema with IH group was exposed to cigarette smoke twice a day （once 30 minutes）and intermittent hypoxia circumstance 8 hours/day. Each group was exposed for 8 weeks. At the beginning of 9 weeks,the blood gas analysis was performed in 5 rats selected randomly from each group,and the rest rats were sacrificed and their hearts and lungs were taken. Under light microscope,the lung tissues stained with hematoxylin-eosin（HE）were examined. The lung pathology and the results of blood gas analysis showed that the emphysema with IH rat model was established successfully. The levels of malonaldehyde（MDA）and superoxide dismutase（SOD）in rat myocardium were measured by enzyme-linked immunosorbent assay（ELISA）,and the subunit p22phox mRNA expressions of nicotinamide adenine dinucleotide phosphate（NADPH）-oxidase were detected by real-time reverse transcription-polymerase chain reaction（RT-PCR）. Results Compared with the normal group, the MDA levels and p22phox mRNA expressions were increased obviously in emphysema group, IH group and emphysema with IH group〔MDA（μmol/g）：2.93＆#177;0.54, 3.58＆#177;0.63, 4.51＆#177;0.72 vs. 1.75＆#177;0.56, p22phox mRNA：0.043＆#177;0.004,0.067＆#177;0.015,0.123＆#177;0.016 vs. 0.018＆#177;0.002,all P＆lt;0.05〕,but the activities of SOD were decreased significantly（U/mg：36.07＆#177;4.79,33.51＆#177;7.12,24.29＆#177;5.36 vs. 46.08＆#177;5.12,all P＆lt;0.05）. In emphysema with IH group,the increase of MDA levels and p22phox mRNA expressions and decrease of SOD levels were more remarkable compared with those in emphysema group and IH group（all P＆lt;0.05）. The expression of p22phox mRNA was positively correlated with MDA level（r=0.734,P＆lt;0.001）. Conclusion The myocardial tissue oxidative stress injury in rats induced by emphysema with intermittent hypoxia exposure is more serious than that induced by exposure of either emphysema or intermittent hypoxia alone,NADPH oxidase possibly being the important medium of myocardial cell response to oxidative stress.
出处
《中国中西医结合急救杂志》
CAS
北大核心
2014年第1期22-25,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
天津市自然科学基金资助项目（13JCYBJc22400）
关键词
间歇低氧
肺气肿
呼吸重叠综合征
心肌细胞
氧化应激
Intermittent hypoxia
Emphysema
Respiratory overlap syndrome
Myocardial cell
Oxidative stress
作者简介
通信作者：吴琦，Email：WQ572004@163．com
