摘要
目的分析根腐病三七根内可培养细菌的多样性。方法用牛肉膏蛋白胨培养基分离根腐病三七根内的细菌,经细菌通用引物27F/1492R扩增16S rDNA后,分别用Rsa I和Hin6 I限制性内切酶对扩增产物进行酶切,结合限制性片段长度多态性(RFLP)分析方法和DNA测序技术,对分离自根腐病三七根内的细菌进行初步鉴定。结果根腐病三七根内的细菌分属于8个类群,依占总菌数的比例分别是芽孢杆菌属Bacillus 22.47%、无色杆菌属Achromobacter 5.62%、寡养单胞菌属Stenotrophomonas 5.62%、类芽孢杆菌属Paenibacillus 4.49%、鞘氨醇杆菌属Sphingobacterium 1.12%、苍白杆菌属Ochrobactrum 1.12%、不动杆菌属Acinetobacter 1.12%及肠杆菌科的一些属58.43%。结论泛菌属和芽孢杆菌属是根腐病三七根中的两大优势细菌类群。
Objective To analyze the cultivable bacterial diversity in the roots of Panax notoginseng with root rot disease. Methods Bacterial strains were isolated from the diseased roots of P.. notoginseng using beef extract-peptone medium. The 16S rDNA was amplificated by Primer 27F/1492R, the product was digested by restriction endonuctease Rsa I and Hin6 I, and PCR-RFLP analysis and DNA sequencing technology were used to identify the bacterial strains in the rotting roots ofP. notoginseng. Results The bacteria could be divided into eight groups including Bacillus （22.47%）, Achromobacter （5.62%）, Stenotrophomonas （5.62%）, Paenibacillus （4.49%）, Sphingobacterium（1.12%）,Ochrobactrum（1.12%）,Acinetobacter （1.12%）, and some bacterial generain Enterobacteriaceae （58.43%）. Conclusion The dominant groups in the rotting roots ofP notoginseng are identified as genera of Pantoea and Bacillus.
出处
《中草药》
CAS
CSCD
北大核心
2014年第3期415-419,共5页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30900963)
国家科技支撑项目(2011BAI13B01-02)
云南省中青年学术和技术带头人后备人才项目(2011HB027)
中国科学院"西部之光"人才培养计划项目
作者简介
作者简介：张智慧（1980-），女，助理研究员，主要从事药用植物资源研究。E-mail：ynzhangzhihui@126．com
通信作者李凌飞Tel：（0871）65228661,E-mail：lilingfei1006@gmail．com
