摘要
利用RAPD分子标记技术对22个葡萄品种的亲缘关系进行了分析.研究表明:8个RAPD引物在22个葡萄品种中共扩增出162条带,其中多态性带为121条,多态率高达74%.采用UPGMA聚类分析法构建的系统树,在相似系数为0.425时,将22个葡萄品种分为6类.葡萄品种间的遗传多样性有明显差异.遗传差异小、亲缘关系近的品种很早就聚在一起,如‘黑大粒’和‘前项无核’;而有些品种,如‘藤稔’和‘高妻’,虽然同属巨峰系葡萄且均为同母本的杂交后代,聚类分析却分属于2大类,说明有性杂交的基因重组可表现出不同的基因型.
Twenty-two genotypes of grapes （Vitis L.）were analyzed with RAPD.The results showed that eight RAPD primers could be amplified 162 bands in 22 grape varieties.There were 121 polymorphic bands,which occupied as high as 74%.When the similarity coefficient was 0.425,the phylogenetic tree constructed by UPGMA cluster analysis could divide the 22 grape varieties into 6 categories.The genetic polymorphism of grape was obvious.The cultivars which owned small genetic differences and close genetic relationship were clustered together early like ‘Exotic’and ‘Pre-entry Seedless’;some cultivars,such as‘Takatsuma’and ‘Fujimineri’,were hybrid parentage having the same female parent,at the same time, they belonged to ‘Kyoho’grapevine series.But they were clustered into two classes.It is suggested that on the condition of hybrid parentage,the crossed progeny could express disparate genotype.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2013年第6期82-87,共6页
Journal of Gansu Agricultural University
基金
甘肃省科技支撑计划(090NKCAO58)
关键词
葡萄
RAPD
亲缘关系
聚类分析
grape
RAPD
genetic relationship
cluster analysis
作者简介
丁洁菲（1987-），女，硕士研究生，研究方向为园艺植物生物技术．E-mail：jaifei_2006@163．com
通信作者：陈佰鸿，男，教授，博导，研究方向为园艺植物生物技术．E-mail：bhch@gsau．edu．cn
