摘要
目的探讨表没食子儿茶素没食子酸酯(EGCG)对鼻咽癌细胞及STAT3表达的影响,揭示EGCG抗鼻咽癌的作用机制。方法体外培养低分化鼻咽癌细胞株CNE2并以不同浓度(0、40、80、160 mg/L)EGCG处理,MMT法检测各浓度EGCG处理组细胞增殖抑制率,Hochest33258染色检测EGCG作用后CNE2细胞的凋亡情况,半定量逆转录PCR(RT-PCR)检测细胞中STAT3 mRNA的表达变化。结果随着EGCG作用浓度的升高与作用时间的延长,CNE2细胞的生长增殖呈浓度-时间依赖性被抑制(P<0.05);CNE2细胞中出现了典型的细胞凋亡小体;随着EGCG浓度的升高,细胞凋亡率亦随之增加;随EGCG浓度和作用时间增加,STAT3 mRNA表达明显下调(P<0.05)。结论 EGCG抑制CNE2细胞增殖,诱导其凋亡,可能与其呈浓度与时间依赖性下调STAT3mRNA的表达相关。
Objective To study the effects of epigallocatechin gallate（EGCG） on nasopharyngeal carcinoma cells and expression of STAT3, and thus to further investigate the mechanism of EGCG in antinasopharyngeal carcinoma. Meth- ods CNE2 cells were treated by EGCG of different concentrations. MTT, Hoechst33258 staining and RT - PCR were introduced for assessment of the growth, apoptosis and STAT3 mRNA of CNE2 cells, respectively. Results Significant inhibition on CNE2 cell proliferation by EGCG was observed in concentration - and time - dependent manners （ P 〈 0. 05 ）. The typical apoptosis bodies in CNE2 cells were observed. As the increase of EGCG concentration, the number of apoptotie cells was significantly increased （ P 〈 0. 05 ）. The expression of STAT3 mRNA was also significantly down - regulated by EGCG in concentration - and time - dependent manners （ P 〈 0. 05 ）. Conclusion EGCG inhibits the proliferation and in induces the apoptosis of CNE2 cells, probably via the induced down - regulation in STAT3 mRNA.
出处
《广东医学》
CAS
CSCD
北大核心
2013年第23期3543-3546,共4页
Guangdong Medical Journal
基金
广西桂林市科学研究与技术开发项目(编号:20120121111)
关键词
EGCG
STAT3
鼻咽癌
凋亡
epigallocatechin gallate
STA33
nasopharyngeal carcinoma
apoptosis
作者简介
通信作者。E—mail：272686181@qq．com
