摘要
禾谷丝核菌侵染小麦引起的纹枯病是一种严重的土传病害,已成为中国小麦高产稳产的重要威胁。为进一步开展禾谷丝核菌的致病机理等研究,通过对酶种类、菌丝酶解时间及渗透压稳定剂等因素进行原生质体制备条件的优化,进一步尝试了PEG-原生质体转化和土壤农杆菌介导的转化方法进行禾谷丝核菌的转化。建立了禾谷丝核菌高效、稳定的原生质体制备体系,通过形态学、生长速率及致病性的比较发现原生质体再生菌株和原始菌株之间差异不显著。多次尝试两种转化方法,没有获得禾谷丝核菌的遗传转化子。原生质体制备体系的获得为禾谷丝核菌遗传转化、原生质体融合等体系的建立奠定了基础。
Wheat sharp eyespot caused by Rhizoctonia cerealis Van der Hoeven, is a soil-borne disease, which has significantly threatened the high and stable production of wheat in China. In order to study the pathogenesis of R. cerealis, the enzyme, the enzymolysis time and the osmotic stabilizer for protoplast preparation were optimized. Two means the PEG- protoplast and Agrobacterium tumefaciens mediation were emloyed to transform R. cerealis. An efficient and stable system of protoplast preparation for R. cerealis was developed, and there was no difference between regenerated strains and original strain in morphology, speed of growth and pathogenicity. No transformant was obtained by both methods. The protoplast preparation system for R. cerealis provided a basis for genetic transformation, protoplast fusion and so on.
出处
《江苏农业学报》
CSCD
北大核心
2013年第6期1313-1319,共7页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(30900928)
小麦产业技术体系建设项目(nycytx-03)
关键词
禾谷丝核菌
原生质体
再生菌株
遗传转化
Rhizoctonia cerealis
protoplast
regenerated strain
genetic transformation
作者简介
任向荣（1987-）,女,山东巨野人,硕士研究生,从事植物病原真菌致病机理等方面研究.（E-mail）renxr_love@126.com
陈怀谷，（E-mail）huaigu@hotmail．com
通讯作者：于汉寿，（E—mail）yuhans@njau．edu．cn；
