摘要
目的 观察人参皂苷Rg1对 β 淀粉样肽 [β AP ,(2 5 - 35 ) ]所致小鼠拟阿尔茨海默 (AD)学习记忆功能障碍的改善作用及其作用机制。方法 小鼠侧脑室注射凝聚态 β AP 4nmol,次日 ,ipRg15和 10mg·kg-1,10d后 ,测试各组被动回避、空间学习记忆能力 ,及皮层、海马组织胆碱乙酰转移酶 (ChAT)和乙酰胆碱酯酶 (AchE)活性变化。结果人参皂苷Rg1可明显改善 β AP所致小鼠被动回避、空间学习记忆能力及皮层海马组织ChAT活性的下降。β AP对小鼠AchE活性无显著性影响 ,但与对照、模型组相比 ,Rg1明显抑制AchE活性。结论 Rg1对 β AP(2 5 - 35 )所致的小鼠学习记忆障碍有显著改善作用 ,其对胆碱能系统的影响是Rg1重要作用机制之一。
AIM To study the effect of ginsenoside Rg 1 on the learning and memory impairment in mice induced by aggregated β AP(25-35). METHODS Mice were administered Rg 1 (5, 10 mg·kg -1 , ip) for 10 d and control mice received daily ip injections of saline after the intracerebroventricular injection of aggregated β AP(25-35). After the final treatment, passive avoidance and performance in the Morris water maze (MWM) were assessed. and the activity of cortical and hippocampal ChAT and AchE were detected after the final behavior test. RESULTS Ginsenoside Rg 1 (5, 10 mg·kg -1 , ip) significantly ameliorated the learning and memory impairment induced by β AP(25-35). Rg 1 (5, 10 mg·kg -1 ) decreased the latencies and swim distances of mice to reach a hidden platform and improved the corresponding changes in search strategies occurred in the Morris water maze, and Rg 1 (10 mg·kg -1 , ip), increased step through latencies also. Biochemical analysis showed that Rg 1 (5, 10 mg·kg -1 , ip) prevented the cortical and hippocampal ChAT activity decline induced by β AP(25-35), and showed inhibition of the activity of AchE, although β AP(25-35) showed no effect on the cortical and hippocampal AchE activity. CONCLUSION These data showed that ginsenoside Rg 1 significantly improved the learning and memory impairment induced by β AP(25-35), and this effect could be attibuted to its inhibition of AchE and increase of ChAT activity.
出处
《药学学报》
CAS
CSCD
北大核心
2001年第1期1-4,共4页
Acta Pharmaceutica Sinica
基金
国家"973"计划基金!资助项目 (g19980 5 110 9)
