摘要
常温下,对两种不同上升流速的IC反应器进行启动实验,通过"葡萄糖配水+OCC造纸废水"的进水方式,逐步提高造纸废水的配比。并研究了两反应器在启动过程中COD去除率、容积负荷(VLR)、污泥比产甲烷活性(SMA)、辅酶F420含量、脱氢酶活性(DHA)的变化差异,并基于荧光原位杂交技术(FISH)对各反应器内功能菌群落分布差异进行表征。结果表明,R1历时27天启动成功,相比种泥食丁酸盐产氢产乙酸菌相对丰度提高了30.76%,耗氢产乙酸菌提高了52.63%,产甲烷菌提高了2.76倍;R2历时57天启动成功,相比种泥R2中的食丁酸盐产氢乙酸菌相对丰度提高了33.74%,耗氢产乙酸菌提高了1.16倍,产甲烷菌提高了2.67倍。耗费启动时间较长但通过降低有效高度从而大大降低加工与维修难度的新型IC反应器R2是可以达到与传统型IC反应器R1相匹敌的处理效能的。
The traditional IC reactor（ R1 ） and the new two point return flow IC reactor（R2） whose rise velocity was about 1/2 of the traditional IC reactor＇ s in the same other conditions was started up, under normal temperature. By the way of entering water of ＂composed water by glucose ＋ OCC waste water＂, the ratio of OCC waste water to composed water could be gradually increased. COD removal rate, volumetric loading rate （VLR） , specific methanogenic activity （SMA）, coenzyme F420, dehydrogenase activity （DHA） in the two IC reactors in the start-up procedure was researched and fluorescence in situ hybridization （FISH） was used to be a characterization of functional bacteria community distribution in the two reactors. The results show, as the start-up was success, compared with the seed sludge, the abundance of the butyrate-oxidizing acetogenic bacteria Syntrophomonas spp. increases by 30. 76% , the homoacetogenic bacteria Acetobacterium species E. limosum increases by 52.63% , and methanomicrobium methanomierobiales increases by 276% , in R1. And the abundance of the butyrate - oxidizing acetogenic bacteria Syntrophornonas spp. increases by 33.74%, the homoacetogenic bacteria Acetobacterium species E. limosum increases by 116%, and methanomicrobium Methanomicrobiales increases by 267%, in R2. Although R2 took a long time to be started up, reduced the difficulty of machining and maintenance greatly by reducing the effective height. And R2 can match R1 in terms of treatment efficiency.
出处
《造纸科学与技术》
北大核心
2013年第6期91-97,共7页
Paper Science & Technology
基金
广东省战略性新兴产业核心技术攻关项目(2012A032300015)
广东省高等学校高层次人才项目
关键词
IC反应器
OCC废水
荧光原位杂交
脱氢酶活性
辅酶F420
internal circulation reactor（IC）
OCC wastewater
fluorescence in situ hybridization
dehydrogenase
coenzyme F420
作者简介
周轩宇，男，硕士研究生，研究方向：造纸废水的生物处理技术。
