摘要
目的探讨以野百合碱(Monocrotaline,MCT)诱导大鼠右心室心肌肥厚模型的制备方法。方法将SD雄性大鼠随机分为对照组(CON组)和野百合碱给药模型组(MCT组),MCT组以2%MCT按60mg·kg-1剂量一次性腹腔注射后饲养3周建立MCT诱导的右心室肥厚大鼠模型。分别测定CON组和MCT组的以下指标:①通过右心室插管法测定平均右心室内压(mRVP)、右心室内压力最大上升速率(+dp/dtmax)、右心室内压力最大下降速率(-dp/dtmax);②测定右心肥厚指数(RVMI);③HE染色观察右心室心肌组织切片。结果与CON组相比:①MCT组的mRVP及RVMI从显著升高(P<0.01,n=18);②右心室+dp/dtmax显著升高(P<0.01,n=18),右心室-dp/dtmax显著降低(P<0.01,n=18);③MCT组右心室心肌纤维粗大,细胞内肌原纤维数量增多,心肌纤维排列紊乱,细胞核深染,形状不整。结论 MCT诱导3周可成功诱导SD大鼠产生右心室心肌肥厚。
OBJECTIVE To explore the approach of right ventricular hypertrophy induced by Monocrotaline. METHODS The SD rats were randomly divided into control group （CON） and Monocrotaline administration （MCT group）. MCT group, a dose of 50mg . kg-1 IN-MCT was given to SD rats by single intraperitoneaI injection and raised 3 weeks. In both models, We measured： ①Mean Right ventricular systolic pressure （ mRVP）, right ventricular pressure maximum rate of rise （ RV ＋ dp/dt,,. ）, right ventricular pressure maximum rate of descent （ RV-dp/ dtmax）Right ventricular mass index （RVMI） ;③Right ventricular myocardium tissue sections were stained by HE and observed under light microscope. RESULTS In comparison to the CON group：（,~）mRYP and RVMI were markedly elevated （P 〈 0. 01 ） in MCT group ; ②RV ＋ dp/dt.,, were markedly elevated （ P 〈 0. 01 ）, RV-dp/dt.,= were markedly decreased （P 〈 0. 01 ） in MCT group ;（~Right ventricular myocardial fibers become thick, intracellu- lar myofibrils increase in the number of disordered arrangement of the myocardial fibers, the nuclei deeply stained, the shape of the nuclei was not regularity in MCT groups. CONCLUSION 3 weeks injected MCT could induce SD rats suffered right ventricular hypertrophy successfully.
出处
《海峡药学》
2013年第11期40-43,共4页
Strait Pharmaceutical Journal
基金
泉州医学高等专科学校校级重点课题(2011XJ02)
关键词
野百合碱
心肌肥厚
Monocrotaline
eardiac hypertrophy
作者简介
陈慧勤，女（1982．8－）。毕业于福建医科大学。职称：讲师。从事心血管病理生理学研究。联系电话：0595-22136661
