摘要
用 0 .2 mg/ L、0 .4mg/ L、0 .8mg/ L 2 ,4- D,通过 1 4 d左右的时间 ,从 4个小麦品种 (“Alondra”、“扬麦9号”、“扬麦 1 0号”、“扬麦 1 5 8”)的未成熟胚上均诱导出芽簇块。其中 ,以 0 .8m g/ L 浓度 2 ,4- D的诱导率最高 ,部分品种 (Alondra和扬麦 1 5 8)的诱导频率可达 80 %。细胞分裂素对芽簇块的诱导有一定的抑制作用 ,KT的抑制效果尤为明显。芽簇块在含 0 .2 mg/ L、0 .4mg/ L 2 ,4- D的原培养基上可以成苗 ,但需时较长 ,成苗数较少 ;转移到添加 1 m g/ L KT+0 .1 mg/ L IAA的成苗培养基上 1 4 d后 ,每个芽簇块平均成苗 4.4个。如分割成小块 ,成苗效率进一步提高。研究结果表明 ,芽簇块在继代培养基上很难保持微芽状态。
The clustered buds were induced from immature embryos of four wheat varieties on induction medium with 0 2 mg/L,0 4 mg/L or 0 8 mg/L 2,4 D respectively.80% immature embryos from part of varieties generated clustered bud on the medium containing 0 8 mg/L 2,4 D,which showed the highest inducing frequency.Cytokinins,especially KT,could restrain the production of clustered bud.Each clustered bud could product average of 4 4 plantlets after it was transferred to the regeneration medium with 1 mg/L KT and 0 1 mg/L IAA only for 14 days.If the clustered bud was cut into small pieces and delivered to regeneration medium,its propagation effciency would be higher than that of the clustered bud directly transferred into regeneration medium.It was very difficult to maintain the micro bud of the clustered buds on subculture medium.
出处
《江苏农业学报》
CSCD
北大核心
2000年第3期139-142,共4页
Jiangsu Journal of Agricultural Sciences
基金
国家"8 63"高技术发展计划项目
关键词
2
4-D
外源激素
小麦
芽簇块
成苗率
组织培养
2,4-dichlorophenoxyacetic acid
exogenous hormone
wheat
somatic cell
clustered bud
plantlet formation
