摘要
目的了解人端粒酶逆转录酶(hTERT)mRNA在胃癌中的表达情况及其与胃癌侵袭、转移和预后的相关性。方法以52例胃癌患者为研究对象,应用RT-PCR技术检测hTERT mRNA在胃原发癌、癌旁正常胃黏膜及淋巴结转移癌中的表达,并对所有病例进行随访,分析其与胃癌侵袭、转移和预后的相关性。结果 hTERT mRNA在胃原发癌和淋巴结转移癌中表达明显增高,而在癌旁正常胃黏膜中表达极低或不表达,差异有统计学意义(P<0.05)。hTERT mRNA表达在浸润癌,癌浸透浆膜面,浆膜侵犯面积>20 cm2、淋巴结分期(7thTNM分期)晚者(N3)中明显增高(P<0.01)。hTERT mRNA高表达组患者5年生存率为17.41%,低表达组为60.04%,差异有统计学意义(χ2=14.20,P=0.0002)。结论胃癌原发灶中hTERT mRNA表达与其恶性生物学行为有关,是反映其侵袭、转移和预后较好的分子指标。
Objective To study the expression of human telomerase reverse transcriptase （hTERT） mRNA in gastric cancer and to evaluate the correlation between its expression and invasion,metastasis and prognosis in gastric cancer. Methods A total of 52 patients with gastric cancer were selected and followed up.The expression of hTERT mRNA in gastric primary focus,normal gastric mucosa and metastatic lymph node were detected by reverse polymerase chain reaction （RT-PCR）.The correlation between hTERT mRNA expression and tumor invasion,metastasis,and prognosis were assessed.Results The expression of hTERT mRNA in gastric primary focus and metastatic lymph nodes significantly increased,while very low and nearly loss in normal gastric mucosa （P〈0.05）.Among patients with infiltrating type,penetrated serosa,area of serosa involved more than 20 cm^2,and metastatic nodes more than 7,the expression of hTERT mRNA was significantly higher （P〈0.01）.The survival rate of patients with hTERT mRNA higher expression （17.41%） was significantly lower than that with its lower expression （60.04%）,the difference was significant （χ^2=14.20,P=0.0002）. Conclusion Up-regulated expression of hTERT mRNA in gastric primary focus may play an important role in tumor invasion,metastasis and poor prognosis,and it is a good molecular marker to reflect the biological behaviors of gastric cancer.
出处
《中国当代医药》
2013年第35期4-6,共3页
China Modern Medicine
基金
国家自然科学基金(81272716)
辽宁省社会发展攻关计划(2012225016)
关键词
胃癌
人端粒酶逆转录酶
侵袭
转移
预后
Gastric cancer
Human telomerase reverse transcriptase mRNA
Invasion
Metastasis
Prognosis
作者简介
通讯作者
