摘要
目的建立通络糖泰颗粒的质量标准。方法采用薄层色谱法对制剂中黄连、芥子、玄参进行定性鉴别，采用高效液相色谱法测定通络糖秦颗粒中盐酸小檗碱含量。结果黄连、芥子、玄参薄层色谱斑点清晰，专属性强，阴性对照无干扰。盐酸小檗碱进样量在0．03729～2．6103μg范围内呈良好的线性关系，r=1．0000，平均回收率为98．85％，RsD；0．70％（n=6）。结论该方法灵敏、简便、准确、重复性好，可作为通络糖泰颗粒的质量控制标准。
Objective To establish the quality standard for Tongluo Tangtai Granules. Methods TLC was applied to the qualitative identification of Rhizoma Coptidis, Semen Sinapis Albae and Radix Scrophulariae, and HPLC was applied to the quantitative determination of berberine in the prescription. Results Rhizoma Coptidis, Semen Sinapis Albae and Radix Scrophulariae could be detected by TLC, the spots were clear and there was no interference for the negative sample. A good linearity of berberine was showed in the range of 0.037 29-2.610 3 μg （r= 1.000 0）. The average recovery of berberine was 98.85%, and RSD was 0.70% （n=6）. Conclusion The methods are simple, sensitive, accurate with good reproducibility. It can be used for the quality control of Tongluo Tangtai Granules.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第12期39-41,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家中医临床研究基地建设医院制剂研发-糖尿病临床基地业务能力建设项目(2012-pp-24)
作者简介
通讯作者：宋英，Tel：028-87783735
