摘要
研究丙烯酰胺（AA）在铂电极上的电化学反应过程以及微分脉冲法测定油炸食品中的丙烯酰胺的含量。以2×10^-3mol／LH2SO4＋0．1mol／LLiCl溶液为电解质时，丙烯酰胺在铂电极上的循环伏安图表明，在-0．58V出现还原峰，在-0．38V处出现氧化峰，并且峰形良好；利用微分脉冲伏安法对油炸食品中的丙烯酰胺进行测定并对实验条件进行优化，扫描范围0～-1．0V、电位增量0．005V、脉冲幅度0．05V、脉冲宽度0．05s、脉冲间隔2．0s、灵敏度100gA，电解质为2×10～mol／LH2SO4＋0．1mol／LLiCl溶液。丙烯酰胺还原峰电流与其浓度在3．0X10～～9．0X10^-8mol／L范围内呈良好的线性关系，其线性方程y=440．95x--1．4893（r=0．9954），加标回收率为94．4％～103．2％，相对标准偏差（RSD）为0．324％-1．785％，最低检测限（LOD）为1．0X10^-6mol／L。与高效液相色谱法相比，该法灵敏度高、操作简便、无需复杂的前处理、设备价格便宜，可用于油炸食品中丙烯酰胺的快速测定。
The electrochemical reaction of acrylamide （AA） at a platinum electrode was studied and an analytical method for the determination of AA in fried foods was proposed by differential pulse voltammetry （DPV）. The cyclic voltammo- gram of acrylamide at the platinum electrode in an electrolyte solution containing 2 X 10-3 mol/L H2SO4 and 0.1 mol/L LiC1 showed one well-defined reduction peak at --0.58 V and oxidation peak at --0.38 V. The optimal experimental conditions for determining acrylamide by DPV were determined as follows： scanning range 0--- 1.0 V, pulse increment 0.005 V, pulse amplitude 0.05 V, pulse width 0.05 s, pulse interval 2.0 s, and sensitivity 100 ktA. A linear relationship was found between the reduction peak current of acrylamide and its concentration in the range of 3.0 X 10-8-9.0 X 10-8 mol/L, with the linear equation of y = 440.95x--1.4893 （correlation coefficient 0.9954）. The recovery rate of AA was 94.4%-103.2% with RSD of 0.324%-1.785%, and the limit of detection （LOD） was 1.0 × 10-8 mol/L. Compared with the current HPLC method, the DPV method was more sensitive without any complicated pre-treatments or expensive equipment. Therefore, the developed method is applicable for the rapid determination of acrylamide in fried foods.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第22期154-159,共6页
Food Science
基金
国家自然科学基金面上项目(20876165)
关键词
丙烯酰胺
油炸食品
微分脉冲伏安法
快速检测
acrylamide
fried food
differential pulse voltammetry
rapid determination
作者简介
张文玲（1988-），女，硕士研究生，研究方向为食品科学。E-mail：zwl_305@163．corn。
通信作者：李书国（1969-），男，教授，博士，研究方向为粮油食品科学与安全技术。E—mail：lishugu0602@126．com
