摘要
目的探讨Survivin和Caspase-3在结直肠癌中表达的临床意义,研究两基因与结直肠癌生物学特性之间的联系及两基因之间的关系。方法应用RT-PCR实验技术测定60例结直肠癌组织及40例癌旁正常组织标本中Survivin和Caspase-3mRNA的表达,分析两基因与肿瘤临床特性、生物学特性之间的相关性及其间的关系。结果 Survivin在结直肠癌中的表达较癌旁正常组织明显升高(P<0.05),而在癌旁正常组织中未能检测到Survivin mRNA。在癌组织中,Caspase-3的表达较正常组织明显降低(P<0.05)。Survivin的表达与患者的性别、肿瘤大小、部位及组织分化程度无明显相关性,但与Dukes分期及有无淋巴结转移密切相关;而Caspase-3的表达仅与肿瘤的分化程度有关,且两基因呈负相关。结论 Survivin与Caspase-3在结直肠癌组织中表达均与癌旁正常组织存在差异,并与肿瘤的Dukes分期、淋巴转移和分化程度有关。因此我们可以推断Survivin可能与肿瘤的侵袭性有着密切的联系;Caspase-3与肿瘤的恶性程度有关。单独或者联合检测两基因mRNA表达可以为临床治疗提供重要的参考依据。
Objective To investigate the level of Survivin and Caspase-3 in human colorectal cancer and relation- ship of the two, and more realationship of clinicopathological characteristics. Methods We sensed mRNA of Survivin and Caspase-3 in 60 human colorectal cancer tissue and 40 adjacent normal colorectal tissue with RT-PCR. Results The expressive levels of Survivin in 60 colorectal cancer samples raised obviously ,but we observed nothing in adja- cent normal colorectal tissue. The expression of Survivin showed no significant relationship to the sex of patients, as the same as the size, location and tissue differentiation grade of cancers, but closely related to Dukes stage and pres- ence or absence of metastasis in lymph node. Caspase-3 just related to the differentiation grade of cancers. Conclu- sion Survivin and Caspase-3 has changed in colorectal compared with comparison. And closely related to the some characteristics of cacer, such as Dukes stage, metastasis in lymph node and differentiation, so we can concluded that Survivin might play an ortant role in chappow, and Caspase-3 might have something to do with malignant stage. Thus we can get some messages of patients by testing it.
出处
《结直肠肛门外科》
2013年第4期224-228,共5页
Journal of Colorectal & Anal Surgery
作者简介
通讯作者
