摘要
对采自伊朗阿塞拜疆东部西红柿大田的棉铃虫Helicoverpa armigera核型多角体病毒3个分离株[Maragheh(MRG),Nebrin(NBN)和Marand(MRD)]繁殖的3个主要参数(幼虫期、接种剂量和培养温度)进行了研究,以在室内条件下筛选很有前景的分离株。在测试的各龄幼虫中,5龄初期幼虫的病毒产量最高。最适接种剂量和接种温度分别为1 965.87 OB/mm2和25℃。而在所有测试中,采自Nebrin的NBN分离株在规模化生产中表现最佳。
Three main parameters of virus propagation, viz., larval stage, inoculation dose and incubation temperature were studied in three nucleopolyhedrovirus isolates of Helicoverpa armigera, i. e., Maragheh （MRG）, Nebrin （NBN）, and Marand （MRD）, collected from tomato fields of East-Azarbaijan, Iran, to select the highly promising isolate under laboratory conditions. Among the larval stages evaluated, the early 5th instar larvae recorded the maximum virus yield. The optimum inoculation dose as well as incubation temperature have been determined as 1 965.87 OB/mm2 and 25℃, respectively. However, the NBN isolate collected from Nebrin recorded the optimum mass production behavior in all the assays.
出处
《昆虫学报》
CAS
CSCD
北大核心
2013年第10期1229-1234,共6页
Acta Entomologica Sinica
关键词
棉铃虫
核型多角体病毒
规模化生产
产量参数
接种剂量
培养温度
Helicoverpa armigera
nucleopolyhedrovirus
mass production
yield parameters
inoculationdose
inoculation temperature
作者简介
Corresponding author, E-mail： a. mehrvar72@ yahoo, com
