摘要
目的研究阻断BDNF及其受体TrkB对人肝癌(HCC)细胞增殖能力的影响,并探讨二者在HCC发生过程中的作用。方法在人HCC细胞系HepG2和97-H中,采用ELISA方法检测BDNF在培养上清中的分泌水平,采用MTT方法测定BDNF中和抗体、干扰BDNF表达或受体酪氨酸激酶(trk)活性抑制剂K252a对细胞增殖的影响。结果 BDNF在HepG2和97-H细胞培养上清中的浓度分别为(88.56±7.45)pg/mL和(119.08±6.21)pg/mL。特异性siRNA转染细胞后,BDNF在HepG2和97-H细胞培养上清中的浓度分别为(73.29±3.63)pg/mL和(102.40±4.01)pg/mL。BDNF中和抗体、干扰BDNF表达或K252a都能有效抑制HepG2和97-H细胞增殖。结论 BDNF/TrkB可能在促进HCC细胞生长方面具有重要作用,有助于HCC的发生发展。
[ Objectives ] To investigate the effects of blocking BDNF and its receptor TrkB on the proliferation of human hepatocellular carcinoma （HCC） cells, and explore the relationship between carcinogenesis and their roles in liver. [ Methods ] ELISA was performed to detect the BDNF secretory levels in supematant of HCC cells HepG2 and 97-H. MTT was used to evaluate the effects of BDNF neutralizing anti-body, interfering with BDNF expression, or tyrosine kinase （trk） inhibitor K252a on cell proliferation. [Results ] BDNF content in supernatant of HepG2 and 97-H cells was （88.56±7.45）pg/mL and （119.08±6.21）pg/mL, respectively. BDNF content in supernatant of HepG2 and 97-H cells transfected with specific BDNF-siRNA was （73.29±3.63）pg/mL and （102.40±4.01）pg/mL, respec- tively. When treated with BDNF neutralizing anti-body, BDNF-siRNA or trk inhibitor K252a, cell proliferation was inhibited significantly. [ Conclusions] BDNFFFrkB may play important roles in supporting cell growth and promoting HCC progression.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第27期54-57,共4页
China Journal of Modern Medicine
基金
2011年辽宁省教育厅科学研究一般项目(No:L2011136)
作者简介
[通信作者]梁健，E—mail：liangj63110@vip．sina.com，Tel：13342452000
