摘要
以100份茄子种质资源中筛选出的耐低温基因型材料E659和低温敏感基因型材料E646为试材,研究了低温处理对不同耐寒性茄子叶片的光抑制能力、丙二醛含量、电导率、过氧化氢酶(CAT)、抗坏血酸酶(APX)和超氧化物歧化酶(SOD)活性的影响。结果表明,在4℃处理5 d后,与低温敏感材料E646相比,低温胁迫下筛选获得的极耐低温基因型材料E659,F V/F M比值下降较少,表明其PSII系统受损较少,而丙二醛含量和电导率上升幅度较低则表明膜脂过氧化反应引起的氧化损伤较轻。4℃低温处理24h之内,发现E659植株中抗坏血酸酶活性增高,过氧化氢酶和超氧化物歧化酶的活性损害减弱或时间推迟。可见,茄子耐低温基因型材料E659在低温胁迫下能快速启动应激反应,增强氧化系统清除能力,减轻低温对植株生长发育的伤害。
In the study, we obtained a low-temperature tolerant cuhivar E659 and a cold sensitive cuhivar E646 by screening from 100 eggplant germplasm resources under low temperature stress. And the two cultivars were measured the photochemical capacity, oxidative damage, hydrogen peroxide accumulation, and antioxidant enzyme activities. The results showed that compared with E646, E659 had less decrease of Fv/FM ratio and less increase of malondialdehyde（MDA） content and electrolyte leakage after 4° treatment for 5days,which suggestted demonstra- ting minor damage of PSII system and oxidative damage by lipid peroxidation in this cuhivar, respectively. The a- scorbate peroxidase （APX） of E659 increased and atalase （CAT） activity superoxide dismutase （SOD） reduced or delayed within 24 hours of 4℃ treatment. These conclusions indicated that the low-temperature tolerant cultivar E659 could possesses a rapid response mechanism and had higher reactive oxygen species system（ROS） clearance capacity to lower cold injury.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2013年第6期1161-1166,共6页
Journal of Plant Genetic Resources
基金
"十二五"农村领域国家科技计划项目(2012AA100103)
浙江省重大科技项目(2012C12903)
浙江省农科院创新提升工程项目(2012R23Y01E01)
关键词
茄子
低温胁迫
光氧化反应
生理机制
eggplant
low temperature stress
photoxidative
physiological mechanism
作者简介
第一作者研究方向为茄子育种与种质创新。E-mail：baocl@126．com
通信作者：毛伟海，研究方向为蔬菜育种与种质创新。E—mail：maowh@126．com
