摘要
假单胞菌(Pseudomonas sp.)YT3和枯草芽孢杆菌(Bacillus subtilis)DZ1是从植物根际土壤筛选出的具有应用潜力的植物根际促生细菌。研究发酵罐中接种量、转速、通气量等因素对YT3菌量以及对DZ1芽孢率、菌量的影响。结果表明:不同的接种量、转速和通气量对Pseudomonas sp.YT3的活菌数、Bacillus subtilis DZ1的活菌数及芽孢率均有显著影响。1 0%的接种量、2 m 3/h的通气量对YT3和DZ1菌株的作用效果与15%的接种量、3 m 3/h相当,但显著高于5%的接种量和1 m 3/h。当转速为200 r/min时,YT3具有最高的活菌数,DZ1具有最高的活菌数和芽孢率。综合分析认为,YT3和DZ1发酵罐的最佳条件为接种量10%、转速200 r/min和通气量2 m 3/h。
Pseudomonas sp. YT3 and Bacillus subtilis DZ1, isolated and screened from the plant rhizo-sphere soil, were plant growth-promoting rhizo-bacteria exhibiting broad application prospects in the bio-fertilizer production. Taking YT3 and DZ1 bacterial strains as research objects, the experiment were conducted to study the influence of inoculation quantity, rotating speed and minute ventilation on the bacteria population and spore rates of YT3 and DZ1 in the fermentation cylinders. The results indicated that different inoculation quantity, rotating speed and minute ventilation could have significantly effects on the bacteria population and spore rates of YT3 and DZ1. Effects of the 10% inoculation quantity and 2 m3/h ventilation were similar on the bacteria population and spore rates of YT3 and DZ1 with that of the 15% inoculation quantity and 3 m3/h ventilation, however, their effects were higher than that of the 5% inoculation quantity and 2 m3/h ventilation. The numbers of YT3, as wellas the numbers and the spore rates of DZ1 reached the highest under 200r/min rotating speed. As a result, the optimum fermentation cylinder conditions of YT3 and DZ1 were 10% inoculation quantity, 200 r/min rotating speed and 2 m3/h ventilation.
出处
《生物灾害科学》
2013年第3期257-261,共5页
Biological Disaster Science
基金
山东省科学技术发展计划项目(2007GG2009007)
关键词
假单胞菌YT3
枯草芽孢杆菌DZ1
接种量
转速
通气量
Pseudomonas sp. YT3
Bacillus subtilis DZ1
inoculation quantity
rotating speed
minuteventilation
作者简介
马海林，男，研究员，主要从事植物营养与肥料方面的研究，E-mail：mahlin@163．com。
