摘要
随着极端微生物及极端酶的广泛研究,嗜酸酶因其在极端酸性环境中具有高的酶活性和稳定性而倍受关注,并取得了较大的研究进展。嗜酸糖苷水解酶是嗜酸酶中最重要的一类,在生物能源、饲料、食品等工业中具有重要的应用前景。综述了重要嗜酸糖苷水解酶,包括嗜酸淀粉酶、嗜酸纤维素酶、嗜酸木聚糖酶和甘露聚糖酶在基因的挖掘、表达、分子改良嗜酸机制研究以及应用等方面国内外的研究进展,展望了嗜酸糖苷水解酶未来可能的研究方向和发展前景。
Extremophiles and enzymes from extremophiles are widely studied. Of them, acidophilic enzyme attracts much attention, due to its high activity and stability under extreme acidic conditions, and this research has made rapid progress. Acidophilic glycosyl hydrolase is one of the most important acidophilic enzymes, and has significant application prospect in bio-energy, animal feed, food and other industries. This paper reviewed the gene cloning, heterologous expression, molecular modification and acidophilic mechanisms of important acidophilic glycosyl hydrolases, including amylase, cellulase, xylanase, and mannanase. The research orientation and development prospects were also elucidated in this paper.
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2013年第5期1-7,共7页
Journal of Agricultural Science and Technology
基金
国家杰出青年科学基金(31225026)
国家863计划项目(2012AA022208)资助
关键词
极端酶
嗜酸菌
嗜酸糖苷水解酶
嗜酸机制
extremozyme
acidophilic microbe
acidophilic glycoside hydrolase
acidophilic mechanism
作者简介
罗会颖，副研究员，博士，主要从事极端酶及酶工程研究。E-mail：luohuiying@caas．cnm
通讯作者：姚斌，研究员，博士，博士生导师，主要从事饲料酶研究。E-mail：binyao@caas．cn
