摘要
在反相ODS柱上,用NH_4Ac-HAc缓冲溶液和甲醇作流动相进行梯度洗脱,清晰地分离了五肽胃泌素及其酶促降解产物。HPLC法分离简便,定量准确,可有效地用于研究活性多肽的酶促降解机制。通过HPLC法探讨五肽胃泌素的降解过程尚属首次。
Pentagastrin and it's enzymatic degradation products can be clearly separated on a reversephase ODS column when eluted gradiently with a solution of NH_4Ac-HAc buffer and methanol.HPLC is a simple, accurate, and efficient separation method for studying enzymatic degradationmechanism of active polypeptide.
关键词
五肽胃泌素
降解
测定
HPLC
胃泌素
Hish Performance liquid Chromatography
penagastrin
degradation
