摘要
目的探讨丙肝病毒核心抗原(HCV Ag)检测对妊娠期早期丙型肝炎诊断的意义。方法对14360例建卡孕妇采用ELISA法检测HCV Ag和丙肝抗体(Anti-HCV),对HCV Ag和/或Anti-HCV阳性者再采用荧光定量PCR法检测丙肝病毒RNA(HCV-RNA)进行确诊。结果 14360例孕妇中有126例感染HCV,感染率为0.88%;HCV Ag检测结果的假阳性率(FPR)明显低于Anti-HCV;Anti-HCV检测的敏感性、特异性和准确性分别为86.5%、60.9%和82.6%,均低于HCV Ag检测的敏感性、特异性和准确性,差异均有统计学意义(P<0.05);HCV母婴传播率为4.8%,母亲HCV病毒量越高者,新生儿越易被传染。结论检测HCV Ag对孕期丙肝早期诊断有较高的特异性、敏感性和准确性,且操作简单,易于推广;同时联合Anti-HCV检测能更高的提高HCV感染筛查准确率。
Objective To discuss the significance of HCV core antigen （Ag） assay in the early diagnosis of hepatitis C for pregnant women. Methods HCV Ag and Anti - HCV were tested by ELISA in 14360 registered pregnant women. For the pro- portion of HCV Ag - and/or Anti - HCV - positive, realtime fluorescent quantitative PCR （RTFQ PCR） was used for quantifi- cation of HCV RNA in confirmatory test. Results The infection rate was 0.88% （ 126/14360） in pregnant women; The false positive rate （FPR） of HCV Ag test was lower than that of Anti - HCV. The diagnostic sensitivity, specificity and accuracy values of the anti - HCV assay were 86.5% , 60.9%, 82.6%, respectively, lower than those of HCV Ag assay （ P 〈 0. 05 ） ; The vertical transmission was 4.8%, the newborn whose mother carried higher viral load was more likely to be infected. Conclusion The HCV Ag assay is highly specific, sensitive, accurate, easy to operate and popularize, so it can be taken as a supplemental test for Anti - HCV assay to increase the accuracy of screening test in the diagnosis of hepatitis C for pregnant women.
出处
《中国卫生检验杂志》
北大核心
2013年第12期2630-2632,共3页
Chinese Journal of Health Laboratory Technology
作者简介
姜大娥（1976-），女，本科，主管检验技师，主要从事医学检验工作。
