摘要
研究探讨了不同的基因型、幼胚取材时期、4℃处理时间、盾片接种方式、分化及生根条件等对小麦幼胚培养再生成株特性的影响 ,并在此基础上建立了一套高效、可靠、重复性好的小麦组培再生系统。优化条件下 ,该系统从幼胚诱导致密愈伤组织的频率为89% ,致密愈伤组织的分化频率诱导 2周时为 95 % ,培养近 3个月时仍可达 5 0 %以上。此外还发现部分叶状结构当转至新鲜的分化培养基上时能够进一步发育成为芽苗。分化的芽苗在生根培养基上大多生成丛生苗。从基部切开后 ,每棵芽苗 /分蘖均可独立成株。
Factors affecting the regeneration of wheat plants from immature embryos, such as differences in genotype, size and development stage of immature embryos, duration of 4℃ treatment, ways of inoculation of immature embryos, differentiation and rooting conditions, etc., were studied. And then a high efficiency regeneration system of wheat plants from immature embryos was established (Fig.2). Under optimal conditions, the frequency of embryogenic callus induction from immature embryos was 89 %, and the frequency of plant regeneration from embryogenic callus was 95% (two week old) and a little more than 50% (three month old), respectively (Table 1). Some leaf like structures continued their development to form shoots when transferred to fresh MSD0 medium, which may be another way of differentiation of wheat callus. Shoots on differentiation medium often developed into clustered shoots, each of which could develop into a whole plant on the rooting medium. All regenerated plants flowered and produced seeds normally.
基金
法国Limagrain公司国际合作项目资助
关键词
小麦
幼胚
组织培养
植株再生
再生频率
wheat, immature embryo, tissue culture, plant regeneration
