摘要
该文在介绍农杆菌介导的番茄遗传转化的影响因素和番茄转基因应用的基础上,重点分析了在农杆菌介导的番茄转化中,影响转化率的农杆菌菌株、载体类型、无菌苗苗龄大小、外植体的类型、预培养的有无、共培养时间的长短、侵染的浓度和时间、抗生素的种类和浓度等重要因素;同时综述了番茄的转基因研究等多种应用价值及其研究成果。
On the basis of an introduction about the infecting factors on agrobacterium-mediated transformation of tomato and its application in transgenic,the important influencing factors such as the strain of agrobacterium,the type of vector, the age of seedling, the kind of explants, pre-culture and co-culture, the density and time of infection, and the type and density of antibiotics in agrobacterium-mediated transformation of tomato were summarized. The application value and achievement of tomato transgenic were reviewed.
出处
《北方园艺》
CAS
北大核心
2013年第20期185-189,共5页
Northern Horticulture
关键词
番茄
农杆菌介导
遗传转化
进展
tomato agrobacterium-mediated
genetic transformation
progress
作者简介
贺玺强（1988-），男，硕士研究生，研究方向为植物分子生物学
徐恒戬（1966-），男，博士，副教授，硕士生导师，现主要从事植物分子生物学与基因功能等研究工作。E—mail：xhj310@163．com
