摘要
目的探讨原花青素对大鼠下肢缺血后血管新生的影响及其相关作用机制。方法将SD雄性大鼠12只随机分为两组：原花青素组和对照组，每组各6只；建立下肢缺血模型，两组每日均灌胃给药，原花青素组给予原花青素200mg／（kg·d），对照组给予同体积生理盐水。于术后第14天取大鼠缺血下肢的下肢内收肌组织，用免疫组织化学法检测CD31在缺血肌肉组织中的表达及新生血管数量，用蛋白印迹法检测低氧诱导因子．1蛋白表达，用酶联免疫吸附试验法测定血管内皮生长因子含量。结果原花青素组缺血肌肉组织中毛细血管计数（169．67±3．11）dVHP明显高于对照组（111．00±3．11）个／HP），差异有统计学意义（t=13．350，P〈0．0001]；原花青素组低氧诱导因子-1蛋白表达（1．90±0．25）明显高于对照组（0．54±0．21），差异也有统计学意义（t=4．183，P=0．0058]；血管内皮生长因子含量（432．86±13．00）μg/L也明显高于对照组（326．68±11．08）μg／L，差异有统计学意义（t=6．216，P〈0．0001]。结论原花青素对缺血后血管新生有促进作用，这一作用可能与原花青素诱导低氧诱导因子-1蛋白表达上调，继而使血管内皮生长因子增加，从而通过多条信号通路诱导新生血管生成增多有关。
Objective To investigate the effect of proanthcyaindin on angiogenesis of rats with ischemic hindlimb. Methods Twelve male SD rats were randomized divided into control group （n = 6） and proanthcyaindin group （PC group, n = 6）. Lower limb ischemia rat model was establish, rats in both group were administration by oral daily, and PC group was given proanthocyanidins 200 mg/（ kg · d）, while the control group received the same volume of saline. Forteen days after surgery, ischemic tissues of adductor were collected for several tests including the expression of CD31 in the ischemic muscle tissue and the number of new blood vessels by immunohistochemical staining, hypoxia inducible factor-1 protein expression by western blot , and vascular endothelial growth factor level test by enzyme-linked immunosorbent assay. Results The capillary count showed that the angiogenesis situation of PC group was （69. 67 ± 3.11 ）/HP , higher than that in control group（111. 00 ± 3. 11 ）/HP, t = 13. 350, P 〈 0.0001 ）. The /-IIF-1 protein expression in PC group was （ 1.90 ± 0. 25 ）, remarkable higher than that in control group （ 0. 54 ± 0. 21, t = 4. 183, P = 0. 0058 ）. Compared with control group,VEGF level in PC group increased（ （432. 86 ±13.00）μg/L vs. （326. 68 ± 11.08）μg/L, t =6. 216,P 〈0. 0001 ）. Conclusion Proantheyaindin plays a positive role in angiogenesis after ischemia. PC may induce up-regulation of HIF-1 and VEGF, and then promote the formation of endovascular through multiple signaling pathways consequently
出处
《中国综合临床》
2013年第11期1179-1181,共3页
Clinical Medicine of China
关键词
新生血管化
缺血
原花青素
低氧诱导因子-1
血管内皮生长因子
Neovascularization
Ischemia
Procyanidins
Hypoxia inducible factor -1
Vascular endothelial growth factor
作者简介
通信作者：段俊丽，Email：duanjunlixh@163．com
