摘要
为快速培育出优良的冰草品种,以蒙农杂种冰草(Agropyron cristatum×A.desertorumcv.Mengnong)幼穗为材料,以拟南芥外源脱水应答转录因子CBF4基因为目的基因,利用基因枪轰击蒙农杂种冰草幼穗诱导愈伤组织,经筛选后获得了抗性转化植株,进一步经PCR和RT-PCR检测得到了670bp左右的电泳条带,表明CBF4基因已在蒙农杂种冰草中得到表达。
In order to speed up the process of wheatgrass germplasm improvement and cultivate more excellent varieties, Agropyron cristatum X A. desertorurn cv. Mengnong＇s young spikes were used as ma- terials, CBF4 was used as target gene and the spike callus were bombarded with gene gun. The regenera- ted plants were screened and tested by PCR and RT-PCR. The electrophoresis band about 670bp showed that the CBF4 gene was expressed in Agropyron cristaturn X A. desertorurn cv. Mengnong. The studies laid a good foundation for breeding new drought-resistance wheatgrass varieties.
出处
《中国草地学报》
CSCD
北大核心
2013年第5期24-28,共5页
Chinese Journal of Grassland
基金
"十二五"国家科技支撑计划项目(2012BAD13B07)
中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)项目(1610332012207)
内蒙古自然科学基金项目(2011MS0402)资助
关键词
蒙农杂种冰草
CBF4基因
遗传转化
抗旱性
Agropyron cristatum X A. desertorum cv. Mengnong
CBF4 gene
Genetic transformation
Drought resistance
作者简介
徐春波（1979），女，内蒙古扎兰屯人，助研，在读博士，主要从事牧草种质资源与育种研究，E-mail：xcb972002@163．com．
通讯作者，E-mail：mfguinm@163．com
