摘要
目的 探讨蛋白含量为300 g/kg高蛋白喂养对宫内发育迟缓（IUGR）大鼠脑髓鞘化过程及学习记忆能力的影响.方法 给予母鼠孕期60 g/kg低蛋白饲料喂养建立IUGR模型,出生后分别予IUGR仔鼠蛋白含量为300 g/kg的高蛋白喂养（SH组）及蛋白含量为210 g/kg的普通饲料喂养（SC组）.正常喂养母鼠所生新生雌鼠40只为正常对照组（CC组）.于第1、7、15、21天测量体质量、脑质量,采用酶联免疫吸附法测定脑组织胼胝体、内囊、外囊的髓鞘碱性蛋白（MBP）水平,第26-32天时行Morris水迷宫实验检测其学习记忆能力,后行脑组织MBP检测.结果 SH组至第21天时,体质量实现了追赶生长,脑质量仍小于CC组（P＜0.05）,但大于SC组；各时间点脑组织MBP表达,SH组、SC组均小于CC组（P均＜0.05）,但SH组表达大于SC组；3组大鼠逃避潜伏期于测试第1-5天逐渐缩短,第5天SH组与CC组逃避潜伏期比较差异无统计学意义（P＞0.05）,SC组逃逸潜伏期明显长于SH组（P＜0.05）,搜寻策略实验显示第5天时SH组和CC组成绩比较差异无统计学意义（P＞0.05）,SC组成绩较CC组和SH组差（P＜0.05）,3组穿越平台次数差异无统计学意义（P＞0.05）.结论 300 g/kg高蛋白喂养IUGR大鼠在一定程度上可促进脑质量的增加,脑髓鞘化的过程,并有助于学习能力的恢复.
Objective To explore the influence of 300 g/kg high protein diet on myelination of the intrauterine grow retardation （IUGR） in the rat brain and their learning and memory ability.Methods The pregnant rats were fed with 60 g/kg low protein diet.After birth,the offspring of IUGR group were fed separately by 300 g/kg high-protein diet （SH group） and 210 g/kg normal-protein diet（SC group）.Forty newborn female pups,born from pregnant rats fed by normal-protein diet,were used as the control group（CC group）.On the first,the 7th,the 15th and the 218t day,the body weight,brain weight of the offspring and their dynamics expressions of myelin basic protein（MBP） in corpus callosum,internal capsule and external capsule were observed with immunohistochemistry method.Morris water maze test was performed to assess learning and memory ability during the 26th-32ed,then the expression of MBP in the brain was observed.Results On the 21st day,the SH group gained catch-up growth weight,while the brain weight of them was still lower than that of the CC group （P ＜ 0.05）,but higher than that of the SC group.At each time point,the MBP expression in corpus callosum,internal capsule and external capsule of SH group and SC group was lower than that of the CC group（all P ＜0.05）,whereas the expression of myelin basic protein in the SH group was higher than that in SC group.The average latencies in all rats were reduced to some degree from the first to the 5th day.On the 5th day,there was no significantly difference between the SH group and CC group （P ＞ 0.05）,while the escape latency was significantly shorter in rats of SH group than those of SC group （P ＜ 0.05）.As far as search strategy was concerned,there was no difference in SH group and SC group of rats（P ＞ 0.05）,but they performed better than those in the SC group on the 5th day（P ＜0.05）.The frequency of passing through the platform quadrant was not significantly difference in rats among the 3 groups （P ＞ 0.05）.Conclusions 300 g/kg high-protein diet on IUGR rats can increase brain weight and the expression of MBP,and improve the ability of learning to some extend.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2013年第17期1351-1354,共4页
Chinese Journal of Applied Clinical Pediatrics
基金
国家自然科学基金（81271378）
苏州市2011年度第六批科技发展计划（SZS201108）
作者简介
通信作者：丁晓春，Email：dxc1225168@sina．com
