摘要
目的:建立HPLC法同时测定草珊瑚药材中反丁烯二酸、新绿原酸、绿原酸、隐绿原酸、异嗪皮啶、落新妇苷、迷迭香酸含量。方法:采用Agilent Zorbax Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),流动相A为乙腈-甲醇(1∶9),流动相B为0.5%的甲酸水溶液,梯度洗脱(0～20 min,20%A;20～30 mim,20%A→30%A;30～60 min,30%A),流速为0.8mL·min–1,检测波长为228 nm(检测反丁烯二酸)、344 nm(检测新绿原酸、绿原酸、隐绿原酸、异嗪皮啶、落新妇苷、迷迭香酸),柱温为40℃。结果:反丁烯二酸、新绿原酸、绿原酸、隐绿原酸、异嗪皮啶、落新妇苷、迷迭香酸浓度分别在24.12～180.90、1.01～101.00、1.75～175.04、2.19～219.20、1.27～127.20、1.57～157.08、2.93～193.28μg·mL-1范围内与峰面积呈良好的线性关系,平均加样回收率(n=6)均在93.92%～103.0%范围内,RSD均小于3.0%。结论:该方法准确,灵敏度高,重复性好,适用于草珊瑚药材中7个有效成分的含量测定。
Objective ： To develop an HPLC method for simultaneous determination of seven main bioactive constitu- ents including fumaric acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenin acid, isofraxidin, astilbin, rosmarinic acid in the whole plant of Sarcandra glabra （Thunb.） Nakai. Methods： An Agilent Zorbax Eclipse XDB -C18 column （250 mm×4. 6 ram, 5 μm） was adopted and the column temperature was at 40 ℃. The mobile phase consisted of a water solution containing 0.5% formic acid and acetonitrile - methanol （ 1 ： 9） in gradient mode [ 0 -20 min ,20% acetonitrile - methanol （ 1：9 ） ;20 - 30 mira, 20%→30% acetonitrile - methanol （ 1：9 ） ;30 -60 min ,30% acetonitrile -methanol （1： 9）], the flow rate was 0. 8 mL . min-1 ,the detection wavelength was set at 228 nm for detecting fumaric acid and 344 nm for detecting neochlorogenic acid, chlorogenic acid, cryptochloro- genin acid, isofraxidin, astilbin, and rosmarinic acid. Results： The good linearity with peak areas was achieved in the range of 24. 12 - 180.90μg . mL-1 for fumaric acid, 1.01 - 101.00 μg . mL-1 for neochlorogenic acid, 1.75 - 175.04 μg . mL-1 for chlorogenic acid, 2. 19 - 219.20 μg . mL-1 for cryptochlorogenin acid, 1.27 - 127.20μg. mL-1 for isofraxidin, 1.57 - 157.08 μg . mL-1 for astilbin, 2. 93 - 193.2μg . mL-lfor rosmarinic acid. The average recoveries（ n = 6） were 93.92% - 103.0% with RSD less than 3.0%. Conclusion： The meth- od developed is accurate, sensitive, reproducible, which can be used for the determination of the seven bioactive constituents in the whole plant of S. glabra.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第9期1512-1517,共6页
Chinese Journal of Pharmaceutical Analysis
基金
福建省科技厅重点项目(2011Y4005)
福建省科技厅闽澳合作项目(2011I0003)
福建省教育厅项目(JA11138)
关键词
草珊瑚
肿节风
活性成分
有机酸
反丁烯二酸
新绿原酸
绿原酸
隐绿原酸
异嗪皮啶
落新妇苷
迷迭香酸
高效液
相色谱
Sarcandra glabra
Sarcandrae Herba
active constituent
organic acid
fumaric acid
neochloro-genic acid
chlorogenic acid
cryptochlorogenin acid
isofraxidin
astilbin
rosmarinic acid
HPLC
作者简介
通讯作者Tel：13799399596；E—mail：fafulyc@126．com.
