摘要
目的分析眼科患者感染的病原菌分布及对抗菌药物的敏感性,为临床医师合理用药提供指导。方法对2011-2012年120例患者眼部感染病原菌,依照微生物临床常规鉴定程序和全国临床检验操作规程操作进行分离培养、鉴定,采用法国生物梅里埃公司生产的全自动鉴定及药敏分析仪VITEK-32鉴定,药物敏感判断采用美国临床实验室标准化委员会的判定标准执行。结果 2011-2012年眼科患者眼部分泌物检出病原菌94株,其中革兰阳性菌44株占46.81%,革兰阴性菌27株占28.72%,真菌23株占24.47%,排名前5位病原菌依次为表皮葡萄球菌、铜绿假单胞菌、金黄色葡萄球菌、流感嗜血菌、曲霉菌属,分别占28.72%、14.89%、13.83%、12.77%、11.70%,革兰阳性菌明显高于革兰阴性菌和真菌(P<0.05);金黄色葡萄球菌和表皮葡萄球菌对万古霉素、利奈唑胺的敏感性均为100.00%,铜绿假单胞菌对哌拉西林、阿米卡星的敏感率为100.00%。结论目前眼部感染病原菌耐药性在逐渐增强,临床治疗中应了解患者眼部感染的病原菌分布,通过药敏试验掌握常见病原菌的药敏性,根据药敏结果合理使用抗菌药物,使患者减少痛苦,控制眼部疾病的发展。
OBJECTIVE To analyze the distribution and drug susceptibility of pathogenic bacteria causing infections in patients of ophthalmology department so as to guide the reasonable medication. METHODS A total of 120 cases of patients with eye infections who were treated in the hospital from 2011 to 2012 in the hospital were enrolled in the study, then the isolation and identification of the pathogens isolated from the patients were performed by referring to the clinical routine identification procedures for microorganism and the national clinical laboratory procedures, the automatic identification and drug susceptibility analyzer VITEK-32 of BioMerieux, France was employed, finally the drug susceptibility was determined by referring to the standards of Committee for Clinical Laboratory Standards. RESULTS A total of 94 strains of pathogens were isolated from the eye secretions from 2011 to 2012,including 44 （46.81%） strains of gram-positive bacteria, 27 （28.72 % ） strains of gram-negative bacteria, and 23 （24.47%） strains of fungi; the Staphylococcus epidermidis, Pseudomonas aeruginosa, Staphylococcus aureus, Haemophilus influenzae, and Aspergillus spp ranked the top 5 species of pathogens, accounting for 28.72%, 14.89%, 13.83%, 12.77%, and 11.70%, respectively. The detection rate of the gram-positive bacteria was significantly higher than that of the gram-negative bacteria or fungi （P〈0.05）. The drug susceptibility rates of the S. aureus and S. epidermidis to vancomycin and linezolid were 100.00% ; the drug susceptibility rates of the P. aeruginosa to piperacillin and amikacin were 100.00%. CONCLUSION Drug resistance of the pathogens causing the eye infections is increasingly severe, it is necessary for the hospital to understand to the distribution of the pathogens causing eye infections, grasp the drug susceptibility of the common species of pathogens, and reasonably use antibiotics based on the result of drug susceptibility testing so as to relive the patients＇ suffering and control the eye diseases.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2013年第18期4577-4578,4581,共3页
Chinese Journal of Nosocomiology
基金
河北省卫生厅科研基金项目(06247)
关键词
眼部感染
病原菌
药敏试验
Eye infection
Pathogen
Drug susceptibility testing
