摘要
实验旨在研究孕酮对蛋鸡CaBP-d28k基因表达的影响。对产蛋高峰期蛋鸡进行孕酮和孕酮受体拮抗剂(RU-486)处理,利用实时荧光定量PCR技术(QRT-PCR),测定在蛋壳形成过程中输卵管子宫部钙结合蛋白(CaBP-d28k)、孕酮受体(PGR)的表达量;同时对血液中钙离子和孕酮浓度变化进行测定。结果表明:孕酮处理后,血液中钙离子浓度显著降低(P<0.05),输卵管子宫部CaBP-d28k表达量也显著降低(P<0.05);孕酮受体拮抗剂处理后,血液中钙离子浓度显著升高(P<0.05),输卵管子宫部CaBP-d28k表达量极显著升高(P<0.01)。结果提示,在蛋壳钙化过程中,孕酮对CaBP-d28k表达和钙离子转运起负调控作用。
This study was aimed to study the effect of progesterone on CaBP-d28k gene expression during the process of eggshell calcification in laying hens. In the present study, the hens were treated with progesterone or progesterone receptor antagonist （RU-486）during the peaking period of egg-laying. Real-time PCR was used to detect the expression levels of CaBP - d28k and PGR mRNA in the uterus of eggshell formation. Meanwhile, differential levels of serum calcium ion （Ca2 ~）and progesterone concentrations were also detected. Results showed that serum Ca2＋ concentrations in test group, which treated by progesterone, was significantly lower than that in control group（P〈0.05）, and significantly lower expression levels of CaBP-d28k mRNA in the uterus was also detected in test group, when compared with the controls （P〈0.05）. Whereas, in group which treated by progesterone receptor antagonist, the serum Ca2＋ concentrations was significantly higher（P〈0.05）than that in control group, and very significantly higher expression levels of CaBP-d28k mRNA in uterus occurred in this group（P〈0.01）, when compared with controls. It is indicated that progesterone has negative regulation on expression of CaBP- d28k and Ca2＋ transport during eggshell calcification process in hens.
出处
《中国畜牧杂志》
CAS
北大核心
2013年第19期22-25,共4页
Chinese Journal of Animal Science
基金
国家自然基金(31060305)
教育部博士点基金(20106518120003)
作者简介
赵海璇（1986-），女，硕士研究生
通讯作者
