摘要
目的:评价荧光定量PCR技术在结核病诊断中的应用价值。方法:采用荧光定量PCR(FQ-PCR)、涂片法和培养法同时检测580例结核患者、疑似结核患者的痰、胸腹水、脑脊液标本,对检测结果进行比较。结果:荧光定量PCR检测阳性率要高于涂片法和培养法的阳性率,统计分析有显著性(P<0.01)。结论:应用荧光定量PCR方法检测结核杆菌,具有特异、灵敏、简便、快速的特点,可作为结核病诊断的常规方法。
Objective：To investigate clinical value of fluorescence quantitative PCR （FQ-PCR）on detection of mycobacterium tuberculosis complex（MTB）.Methods：580 sputum, cerebrospinal fluid（SCF）,hydrothorax and ascite samples from clinical MTB and suspected patients were detected by FQ-PCR,aeid-fast staining and culture respectively.Sensitivity and specificity of FQ-PCR were assessed by comparing test results.Results：The Positive rate of FQ-PCR in testing samples was higher than that of acid - fast staining and cuhure,and there was a significant difference（P〈0.01 ）.Conclusion：FQ-PCR characterized by distinct specificity, high sensitivity, simplicity and quick-response can be used as a conventional method to diagnose of tuberculosis.
出处
《北方药学》
2013年第10期58-60,共3页
Journal of North Pharmacy
关键词
荧光定量PCR
结核分枝杆菌
涂片法和培养法
Fluorescence quantitative PCR Mycobacterium tuberculosis complex Acid-fast straining
