摘要
目的:探讨健脾增液汤对2型糖尿病大鼠脂肪-胰岛素轴的早期干预作用。方法 :大鼠随机抽以10只为正常组,其余大鼠腹腔注射小剂量链脲佐菌素加高糖高脂饲料建立2型糖尿病大鼠模型,成模后,将模型动物随机分为模型组、二甲双胍组(0.25mg/kg)、健脾增液汤组(18g/kg)、健脾化湿组(9g/kg)、增液汤组(9g/kg),治疗8周,应用放射免疫法检测在大鼠血清瘦素(Leptin)水平、胰岛素(INS)水平,生化方法检测血脂、血游离脂肪酸(FFA)的含量,分析血脂、Leptin、INS在糖尿病发病中的相互关系,并以二甲双胍作为对照,分析了中药健脾增液汤及其拆方对糖尿病脂肪-胰岛素轴的早期干预作用。结果 :(1)各组大鼠血脂中总胆固醇(TC)、甘油三酯(TG)、FFA升高,高密度脂蛋白降低,瘦素水平、胰岛素水平均升高,与正常组有显著性差异。(2)健脾增液汤组(18g/kg)、健脾化湿组(9g/kg)、增液汤组(9g/kg)的高密度脂蛋白(HDL)升高,FFA、胰岛素、瘦素降低,而健脾增液汤组(18g/kg)、健脾化湿组(9g/kg)组的为TG、TC降低显著。结论:各组药物均可改善糖尿病的脂代谢异常,改善脂肪-胰岛素轴的功能,从而对因脂毒性、胰岛素抵抗引起的糖尿病的发生和发展有一定的干预作用。
Objective： To observe the effects of Janpizengye soup on fat-insulin-axis in the obese-rats of type 2 diabetes.Methods： The model of type 2 diabetes was established by intraperitoneal injection of streptozotocin（STZ） at a dose of 30mg / kg and a high-carbohydrate / high-fat diet.Experimental rats were randomly divided into six groups： normal group,model group,metformin group,combination group,invigorating spleen to resolve dampness group,Zengye soup group and they were treated respectively.Eight weeks later,Leptin 、INS、FFA、Lipids contents were determined through radioimmunoassay and Biochemical method respectively.Results ：（1） Total cholesterol（TC）,triglyceride（TG）,FFA and leptin levels、insulin levels were elevated,there is a significant difference with the normal group（P ＆lt; 0.05）（2） After treatment,FFA、insulin、leptin were decreased（P ＆lt; 0.05） in combination group（18g / kg）,invigorating spleen to resolve dampness group（9g/kg）,Zengye soup group（9g/kg）,TG、TC were also decreased in combination group（18g/kg）,invigorating spleen to resolve dampness group（9g / kg）.Conclusion： Invigorating spleen and zengye soup can improve fat-insulin-axis function,which due to improve lipid toxicity,insulin resistance,leptin resistance.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第4期141-143,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
江苏省中管局课题(LZ09096)
南京市卫生局课题(YKK09063)
