摘要
目的:分析结直肠癌相关成纤维细胞(carcinoma associated fibroblasts,CAFs)与正常成纤维细胞(normal fibroblasts,NFs)的微小RNA(miRNA)表达谱差异。方法:收集结直肠癌组织和癌旁正常肠黏膜共3对,抽提、纯化RNA,加入荧光标记后与miRNA寡核苷酸基因芯片杂交,应用SAM软件进行数据分析,对有显著差异的miRNA进行实时荧光定量PCR验证,采用靶基因分析软件分析miRNA功能。结果:hsa-miR-138,hsa-miR-210,hsa-99a等12个miRNA在肿瘤组织中显著上调,中位假基因验出率(FDR)<5%;has-miR-29b、has-miR-494、has-miR-126等28个miRNA显著下调(FDR<5%)。结论:结直肠癌与正常结肠黏膜之间存在明显的miRNA差异表达谱,这些miRNA的差异性表达可能与结直肠癌的发病、侵袭、转移相关。
Objective：To explore miRNAs expression differences between carcinoma associated fibroblasts（CAFs）and normal fibroblasts（NFs） or the patient-to-patient variability in miRNAs expression in coloretal cancer.Methods：We established primary cultures of CAFs and paired NFs from six resected colorectal cancer tissues that had not previously received radiotherapy or chemotherapy treatment and analyzed with miRNAs microarrays.The array data were analyzed using paired SAM t-test and filtered according to α and q values.Pathway analysis was conducted using DAVID v6.7.Results：We identified 12 dysregulated miRNAs in CAFs：three were up-regulated （hsa-miR-138,hsa-miR-210,hsa-99a）,while 28 were down-regulated （has-miR-29b,has-miR-494,has-miR-126）.Their target genes are known to affect cell differentiation,adhesion,migration,proliferation,secretion and cell-cell interaction.Conclusion：By our knowledge it is firstly identify the expression profiles of miRNAs between CAFs and NFs.This study provides a new perspective on CRC metastasis and recurrence.
出处
《现代肿瘤医学》
CAS
2013年第9期1918-1922,共5页
Journal of Modern Oncology
基金
陕西省教育厅自然科学基金项目(编号:12JK0709)
西安医学院博士启动基金项目(编号:2011DOC06)
作者简介
王善伟（1975-）,女,新疆昌吉人,博士,讲师,主要从事肿瘤分子病理学研究.E-mail：xinjiangykx@163.com
【通讯作者】陈丽荣（1959－），男，浙江丽水人，教授，博士生导师，主要从事病理学及肿瘤学研究。
