摘要
以花生蛋白粉为原料,采用Viscozyme L预水解,再分别用Alcalase、Flavourzyme、Protamex、中性蛋白酶和木瓜蛋白酶水解法制备5种花生抗氧化肽,依次简写为AP、FP、PP1、NP和PP2。应用4类体外抗氧化试验:清除DPPH自由基、还原力、铁离子螯合力和抗脂质体过氧化能力,研究了5种抗氧化肽的抗氧化活性。通过综合分析抗氧化活性试验结果,5种抗氧化肽的抗氧化活性大小为PP2>AP>FP>PP1>NP。PP2有最大的清除DPPH自由基活性、还原力和抗脂质体过氧化能力,表明制备抗氧化肽的最佳蛋白分解酶是木瓜蛋白酶。因此,使用木瓜蛋白酶研究和开发抗氧化肽是一种有效地进一步利用花生蛋白的方法。
Use peanut protein powder as the raw material,five kinds of peanut antioxidant peptides（abbreviated as AP,FP,PP1,NP and PP2） were obtained through steps of Viscozyme L pretreatment and Alcalase,Flavourzyme,Protamex,Neutral protease and Papain hydrolysis,respectively.Four types of antioxidant activities evaluation methods in vitro including scavenging of DPPH free radical,reducing power,iron ion chelation and anti-lipid peroxidation were presented to evaluate the antioxidant activities of peanut antioxidant peptides.The order of antioxidant activities of five antioxidant peptides was PP2APFPPP1NP by comprehensive analysis of the antioxidant experimental results.The results indicated that the optimum proteolytic enzyme for preparing antioxidant peptide was papain.Among the five antioxidant peptides,PP2 had the most antioxidant activities of scavenging of DPPH free radical,reducing power,anti-lipid peroxidation.Therefore,the research and development of antioxidant peptide with the antioxidant function by using papain is an effective approach to further exploit peanut protein.
出处
《核农学报》
CAS
CSCD
北大核心
2013年第8期1162-1167,1172,共7页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(31100205)
国家科技支撑计划(2012BAK17B13)
青岛市公共领域科技支撑计划(11-2-3-25-nsh)
作者简介
刘洪对，男，助理研究员，从事植物蛋白肽的开发和应用研究。E—mail：liuhd78@163．com.
通讯作者：于丽娜，女，助理研究员，从事植物蛋白和活性肽开发研究。E-mail：lhtyln0626@yahoo．com．cn
