摘要
从剑麻叶片组织内分离获得6株内生细菌,其中JM-3菌株对离体苹果枝条上苹果腐烂病菌的扩展致病有较好的抑制效果。JM-3菌株在培养过程中能产生抑菌物质,其无菌发酵滤液能抑制腐烂病菌菌丝的生长和分生孢子的萌发,导致菌丝和芽管膨大畸形,最终导致菌丝破裂、原生质外流。JM-3菌株对多种果树和农作物病原真菌菌丝的生长都有明显的抑制作用。经16S rDNA序列分析和RiboPrinter分析,鉴定菌株JM-3为枯草芽孢杆菌。
Six isolates of endophytic bacterial strains were isolated from healthy leaves of Agavesisalana Perrine,in which the strain JM-3 inhibited canker lesion expansion of Valsa ceratosperma on excised twigs significantly.The cell-free fermentation liquid of JM-3 inhibited mycelium growth and conidial germination of Valsa ceratosperma.The isolates produced anti-fungi substances,which caused malformation of mycelia and germtubes.The malformed mycelium burst and protoplasm flow-out finally.JM-3 also showed clear inhibiting effect against mycelial growth of pathogen from several fruit trees and crop.JM-3 was identified as Bacillus subtilis based on 16S rDNA sequencing and RiboPrinter results.
出处
《华北农学报》
CSCD
北大核心
2013年第4期208-213,共6页
Acta Agriculturae Boreali-Sinica
基金
现代农业产业技术体系项目(CARS-28)
山东省科技攻关计划项目(2010GNC10918)
国家自然科学基金项目(31000891
31272001)
山东省"泰山学者"建设工程项目
关键词
内生细菌
枯草芽孢杆菌
苹果腐烂病
Endophytic bacteria
Bacillus subtilis
Valsa ceratosperma
作者简介
张淑颖(1980-),女,山东文登人,在读博士,主要从事病虫害生物防治研究
通讯作者:李保华(1961-),男,山东潍坊人,教授,博士,主要从事植物病害流行学研究。