摘要
为探讨猪尿中β2受体激动剂UPLC—MS／MS检测的基质效应及消除方法，将猪尿液样品经高氯酸酸解，乙酸乙酯和甲基叔丁基醚混合提取后，用MCX固相萃取柱净化，进行超高效液相色谱-串联质谱法检测。发现猪尿液中8种β2-受体激动剂UPLC-Ms／MS检测的基质效应为71．0％～114．5％，并比较基质添加标准曲线以及内标法两种定量方法，对基质效应进行消除研究。结果表明，5．0mL猪尿液在0．4、1．0、2．0ng／mL添加浓度下，内标法的定量结果为64．7％～136．0％。基质添加校正曲线的定量结果为77．9％-117．9％。基质添加校正曲线定量更加稳定，可以取代内标法进行定量。
To investigate the matrix effects in the residue detection of β2-agonists in pig urine and elimina- ting methods, pig urine samples were acidolysised by perchloric acid, extracted with ethyl acetate and meth- yl tertiary butyl ether, the extracts were applied to MCX cartridge for cleanup, the separation was per- formed by ultra performance liquid chromatography-tandem mass spectrometry. 71.0%-114.5%levels of matrix effects were found in 8 β2-agonists detection. The internal standard method and matrix dilution cali- bration curve were compared to eliminate matrix effect. The results showed that,quantitative results of the internal standard method was 64. 7%-136.0%, matrix spiked calibration curve quantitative results was 77.9% -117.9%,with 5.0 mL pig urine at 0.4 ng/mL,1.0 ng/mL and 2.0 ng/mL standard added. The quantitative matrix spiked calibration curve was more stable, and internal standard method could be re- placed by matrix spiked calibration curve for quantitative.
出处
《动物医学进展》
CSCD
北大核心
2013年第9期66-70,共5页
Progress In Veterinary Medicine
作者简介
邱增枝（1986-），男，山东临沂人，硕士研究生，主要从事兽药残留研究。
通讯作者
