摘要
目的研究LED红光[波长(630±15)nm]照射对高脂血症大鼠血脂及肝脏病理学改变的影响。方法 36只雄性SD大鼠,随机分为正常对照组(12只)和高脂模型组(24只)。正常对照组喂饲普通饲料,高脂模型组喂饲高脂饲料,连续喂养6周。造模成功后,将高脂模型组大鼠随机分为高脂对照组(不给予照射)和LED治疗组(每天照射30 min,连续28 d)。分别于照射的第15天和第29天测定TC、TG、LDL-C、HDL-C的含量。于照射第29天处死大鼠,称取肝脏湿重并进行大体及镜下观察。结果 LED照射的第15天后,LED治疗组与高脂对照组相比,血清TC、TG、LDL-C的含量降低,HDL-C的含量升高,但只有LDL-C有统计学意义(P<0.05)。LED照射的第29天,LED治疗组与高脂对照组相比,血清TC、TG、LDL-C的含量明显降低,HDL-C的含量升高,差异有统计学意义(P<0.01)。肝重、肝指数也明显降低(P<0.05)。经LED照射后,LED治疗组大鼠肝脏脂肪变性的程度较高脂对照组有明显改善。结论LED红光照射可以降低高脂血症大鼠血清TC、TG、HDL-C、LDL-C的含量,并对高脂血症大鼠肝脏脂肪变性有一定的治疗作用。
Objective To observe the effects of light emitting diode [LED,（630 ±15）nm] on blood lipid and pathological changes of liver in rats with hyperlipidemia .Methods Thirty-six male Sprague-Dawley rats were randomly divided into control group （ n =12 ） and hyperlipidemia model group （ n =24 ） .The rats in control group was kept on feeding with normal diet ,however ,the rats in hyperlipidemia model group were fed with fat-rich forage for six weeks .After the hyperlipidemia models were established ,the rats were randomly divided into hyperlipidemia control group and LED treatment group .The rats in LED treatment group were given LED red light irradiation for 28 days.The levels of blood lipid including total cholesterol （TC）,triglyceride （TG）,low-density lipoprotein （LDL-C）,high-density lipoprotein （HDL-C） were detected by biochemical assay at the 15th day and 29th day.The animals were sacrificed at the 29th day and their livers were taken out for gross and microscopic examination.Results After 14-day irradiation,the serum levels of TC,TG,LDL-C in LED treatment group were decreased,but the serum levels of HDL-C were significantly increased ,as compared with those in hyperlipidemia control group,and there was a significant difference in serum levels of LDL-C between the two groups （ P 〈0.05）.After treated with LED for 28 days,the serum contents of TC,TG and LDL-C were significantly decreased, while the serum levels of HDL-C were increased significantly , as compared with those in control group （ P 〈0.01）.The liver weight and liver index were also significantly decreased （ P 〈0.01 ）.The degree of fatty degeneration of liver in LED treatment group was obviously relieved ,as compare with that in hyperlipidemia model group.Conclusion LED red light irradiation can significantly decrease blood lipid levels and has certain therapeutic effect on fatty degeneration of liver in rats with hyperlipidemia .
出处
《河北医药》
CAS
2013年第17期2571-2573,共3页
Hebei Medical Journal
关键词
发光二极管
血脂
脂肪肝
高脂血症
大鼠
light emitting diode
blood fat
fatty liver
hyperlipidemia
rats
作者简介
通讯作者：贾子善，050051河北省人民医院康复科；E—mail：jzs1963@163．com
