摘要
目的研究不同剂量血必净注射液对肢体缺血再灌注损伤（ischemia reperfusion iniury，IRI）的治疗效果与机制。方法30只新西兰大耳兔按随机数字表法分为对照组、血必净Ⅰ组和Ⅱ组各10只。采用兔下肢IRI模型，各组恢复血流后予以相应的治疗：血必净Ⅰ组给予4ml／kg血必净注射液和6ml／kg等渗盐水；血必净Ⅱ组给予2ml／kg血必净注射液和8ml／kg等渗盐水；对照组给予等渗盐水10ml／kg。于再灌注前及再灌注后1，2，4h分别采取静脉血样本测定凝血功能：活化部分凝血酶时间（APTT）、纤维蛋白原（Fib）、国际标准比值（INR）、凝血酶原时间（PT）；生化指标：白蛋白（ALB）、血清乳酸脱氢酶（LDH）、血清肌酸激酶（CK）含量。结果血必净I组APrr再灌注后1，4h较对照组有明显改善（P〈0．01）；血必净Ⅰ组、Ⅱ组PT再灌注后较再灌注前有明显延长（P〈0．05）；血必净Ⅰ组再灌注后4hFib含量较再灌注前有显著增高（P〈0．05）；血必净Ⅰ组、Ⅱ组再灌注后1h ALB与再灌注前差异无统计学意义（P〉0．05）；血必净Ⅰ组再灌注后LDH、CK含量显著低于对照组（P〈0．05）。结论血必净注射液能够减轻肢体IRI，且血必净Ⅰ组效果好于血必净Ⅱ组；其机制主要可能与其调节机体凝血功能及减轻肌组织细胞损伤等有关。
Objective To investigate effect and mechanism of Xuebijing administration at various concentrations on isehemia repeffusion injury （IRI） of rabbit limbs. Methods Thirty New Zealand rabbits were divided into control group （n = 10）, Xuebijing group I （n = 10） and Xuebijing group Ⅱ （n = 10） according to random number table. Rabbit models of IRI in lower extremities were established. Each group received corresponding therapy after repeffusion： rabbits in Xuebijing group Ⅰ were firstly administered 4 ml/kg Xnebijing solution and 6 ml/kg isotonic saline; rabbits in Xuebijing group Ⅱ were administered 2 ml/kg Xuebijing solution and 8 ml/kg isotonic saline ; rabbits in control group were simply administered 10 ml/kg isotonic saline. Venous blood samples were collected before reperfusion and at 1 h, 2 h, 4 h after reperfusion to measure coagulation parameters （APTT, Fib, INR and PT ） and biochemical items （ALB, LDH and CK）. Results AVIT in Xuebijing group Ⅰ presented obvious improvement at 1 h and 4 h after reperfusion as compared with control group （P 〈 0. 01 ）. PT in Xuebijing groups Ⅰ and Ⅱ was significantly longer after reperfusion than that before reperfusion （ P 〈 0.05 ）. Fib level in Xuebijing group I was much higher at 4 h after reperfusion than that before reperfusion （P 〈 0.05 ）. ALB level at 1 hour after reperfusion showed no statistical differences from that before reperfusion in Xuebijing groups I and Ⅱ （P 〉 0.05 ）. LDH and CK levels in Xuebijing group Ⅰ were much lower than those in control group after reperfusion （P 〈 0. 05 ）. Conclusions Xuebijing injection relieves limb IRI, with better effect in Xuebijing group I than in Xuebijing group Ⅱ. Therapeutic mechanism may be associated with its involvement in adjusting clotting function and mitigating injury of muscle tissues.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2013年第8期796-799,共4页
Chinese Journal of Trauma
基金
全军“十二五”课题资助项目（AWS11J008）
关键词
缺血
再灌注损伤
治疗
Ischemia
Reperfusion Injury
Therapy
作者简介
张良，电话：13637818469，Email：liangelean@gmail．com
通信作者：周继红，电话：023-68757441，Email：traumazjh@126．com
