摘要
以猪骨粉为原料,水解度(DH)为评价指标,研究不同因素对猪骨粉酶解的影响。在单因素试验的基础上,选用加酶量、温度、起始pH值、酶解时间4个因素,通过四因素三水平的响应面分析法优化猪骨粉的酶解条件。结果表明:猪骨粉与水固液比为1:20(g/mL)时,最佳水解条件为:加酶量5722.25U/g、温度59.12℃、起始pH7.96、酶解时间3.19h,在此酶解条件下,预测水解度可达到17.41%。验证实验水解度为17.13%,表明实验结果与软件分析结果相符。
A response surface experimental design of four variables at three levels each was used to optimize the enzymatic hydrolysis of pig bone meal(PBM) to obtain maximum degree of hydrolysis(DH).Alkaline protease was identified as the best protease for PBM hydrolysis.The optimal hydrolysis conditions were determined as 1:20(g/mL),5722.25 U/g,59.12 ℃,7.96 and 3.19 h for solid/liquid ratio,enzyme dose,temperature,initial pH and hydrolysis time,respectively.The predicted DH under these conditions was 17.41%,which was consistent with the actual value of 17.13%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第16期77-81,共5页
Food Science
基金
安徽省2008年科技攻关计划项目(2008A08010302209)
安徽省战略性新兴产业项目(11010301017)
关键词
猪骨粉
酶解
响应面法
水解度
pig bone meal
enzymatic hydrolysis
response surface methodology
degree of hydrolysis
作者简介
赵妍嫣(1972-),女,副教授,博士,研究方向为发酵工程、农产品深加工。E-mail:zhaoyy33@163.com