摘要
目的:初步探讨黄芪甲苷对心肌缺血心律失常模型大鼠心肌细胞Ca2+浓度的影响。方法:将心肌缺血再灌注模型大鼠随机分为模型组和假手术组,各组给药7d后开始造模。将Fura-2/AM荧光探针负载于大鼠心肌细胞,采用荧光分光光度计检测Ca2+浓度。结果:模型组心肌细胞内游离Ca2+浓度与假手术组比较,差异有显著性(P<0.01);与黄芪甲苷的低剂量组比较,无显著性差异(P>0.05);与黄芪甲苷的中、高剂量组比较,有显著性差异(P<0.01);黄芪甲苷的中、高剂量组与普罗帕酮组比较,无显著性差异(P>0.05)。结论:黄芪甲苷可降低心肌细胞内游离Ca2+浓度,从而减少心律失常的发生,起到抗心律失常的作用。
Objective：To investigate the influence of astragaloside IV on myocardial ischemia arrhythmias in rats about the concen tration of Ca2＋ in myocardial cell. Methods： With rat model of ischemic reperfusion injury was prepared, randomly divided into six groups. After 7 days, the concentration of Ca2＋ was detected by Fura-2/AM fluorescent probes on the fluorescence spectrometer. Results：There was significant statistic difference with intracellular the concentration of free Ca2＋ in model group, compared with the sham group （P〈0.01）, and no significant in astragaloside IV with small dose group （P〉0.05）, and as well as with the astra- galoside IV medium dose group and large dose group （P〈0.01）. And no difference from astragaloside IV with medium and large dose group to propafenone group （P〉0.05）. Conclusion： Astragaloside Ⅳ can reduce the concentration of Ca2＋ in myocardial in- tracellular, and thus plays a role of anti-arrhythmia.
出处
《亚太传统医药》
2013年第8期11-12,共2页
Asia-Pacific Traditional Medicine
基金
广东省中医药强省课题(20121220)
作者简介
作者简介：龚奥娣，女，广东省医药进出口公司主管药师，研究方向为公共卫生。
