摘要
【目的】探讨不同磷浓度胁迫条件下油茶叶片的光合生理参数、光合色素含量、RuBP羧化酶及PEP羧化酶活性等指标的变化规律,为耐低磷油茶品种的选育和高产栽培提供参考。【方法】选择油茶良种"湘林XLC15"和"华硕"为试材进行不同磷浓度沙培盆栽处理,测定不同叶龄的光合生理参数、光合色素含量及RuBP和PEP羧化酶活性,并采用Ducan新复极差法进行各生理指标与净光合速率(Pn)之间的相关性分析。【结果】"湘林XLC15"叶片的净光合速率、气孔导度、蒸腾速率、光合色素含量及RuBP羧化酶活性均在磷浓度为1.0mmol/L时达到最高,而"华硕"则在磷浓度为0.1mmol/L时已达到较高值,且在磷浓度为0.1～1.0mmol/L时变化幅度不大。相关性分析表明,磷胁迫条件下油茶幼苗叶片的气孔导度、蒸腾速率、总叶绿素含量、类胡萝卜素含量、RuBP羧化酶及PEP羧化酶活性与净光合速率呈正相关,胞间CO2浓度、叶绿素a/b与净光合速率呈负相关。【结论】与"湘林XLC15"相比,低磷胁迫条件下"华硕"的光合色素含量较高,RuBP羧化酶活性较强,从而有利于保持较高的净光合速率。
【Objective】 The study was performed to explore the variation of photosynthetic physiological parameters,photosynthetic pigment contents,the enzyme activities of RuBPCase and PEPCase and other indexes of Camellia oleifera Abel leaves under phosphorus stress conditions,to improve breeding and high-yield cultivation of low phosphorus tolerance C.oleifera varieties.【Method】 Using two superior varieties,C.oleifera ＇Xianglin XLC15＇ and ＇Huashuo＇ as experimental materials,sand culture pot experiment with different phosphorus levels were conducted and photosynthetic physiological parameters,photosynthetic pigment contents,the enzyme activities of RuBPCase and PEPCase and other indicators of C.oleifera were measured.Correlation analysis between the various physiological indicators and net photosynthetic rates（Pn） was conducted using the Ducan new multiple range method.【Result】 Net photosynthetic rates（Pn）,stomatal conductance（Gs）,transpiration rates（Tr）,photosynthetic pigments contents and RuBPCase activities of C.oleifera ＇Xianglin XLC15＇ reached the highest values in 1.0 mmol/L phosphorus concentration,whereas those of ＇Huashuo＇ reached higher values in 0.1 mmol/L phosphorus concentration and changed slightly in 0.1-1.0 mmol/L.Correlation analysis showed that stomatal conductance（Gs）,transpiration rates（Tr）,total chlorophyll contents,carotenoid contents,the enzyme activities of RuBPCase and PEPCase of two C.oleifera species were positively correlated to net photosynthetic rates under phosphorus stress,while intercellular CO2 concentrations,chlorophyll a/b and net photosynthetic rates showed a negative correlation.【Conclusion】 Compared with C.oleifera ＇Xianglin XLC15＇,＇Huashuo＇ had higher contents of chlorophyll,stronger activity of RuBPCase,and higher net photosynthetic rate.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2013年第7期125-132,共8页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家"十一五"科技支撑计划项目(2009BADB1B02)
关键词
油茶
磷胁迫
净光合速率
RuBP羧化酶活性
PEP羧化酶活性
Camellia oleifera Abel
phosphorus stress
net photosynthetic rate
RuBPCase activity
PEPCase activity
作者简介
[作者简介]张雪洁（1987-），女，河北石家庄人，在读硕士，主要从事经济林栽培育种研究。E—mail：zxjai@163．com
[通信作者]谭晓风（1956-），男，湖南茶陵人，教授，博士，主要从事经济林栽培育种与林业生物技术研究。E-mail：tanxiaofengcn@126．com
