摘要
目的观察大剂量激素及木豆叶干预后骨髓间充质干细胞(MSCs)的蛋白质变化,探讨木豆叶治疗股骨头坏死的作用机理。方法培养人MSCs,设正常对照组、模型(激素)组及木豆叶含药血清组,通过双向电泳比较正常对照组与模型组、模型组与木豆叶含药血清组的蛋白质表达差异,通过质谱分析对差异蛋白质进行初步鉴定。结果与正常对照组比较,模型组MSCs有38个蛋白点发生显著变化;与模型组比较,木豆叶含药血清组MSCs有28个蛋白点发生显著变化,MCSc中热休克蛋白(HSP)、含缬络肽蛋白(VCP)及COP9表达水平显著升高。结论大剂量激素干预可造成MSCs内脂肪代谢失调、分化异常,木豆叶可抑制MSCs凋亡,并可能通过MAPK途径,影响其成骨分化及修复骨坏死而发挥治疗股骨头坏死的作用。
Objective To investigate the changes of protein expression in bone marrow mesenchymal stem cells（MSCs）treated by large doses of hormones and Folium Cajani,and to explore its possible therapeutic mechanism for femoral head necrosis.Methods The cultured human MSCs were divided into normal control group,hormone-treat model group and group of serum containing Folium Cajani.Two-dimensional electrophoresis was used to compare the protein expression difference in the three groups,and the protein spots with differential expression were analyzed by mass spectrometry.Results A total of 38 protein spots had differential expression in MSCs of the hormone-treat model group as compared with the normal control group.In MSCs of Folium Cajani group,28 protein spots had differential expression as compared with the hormone-treat model group,and heat-shock protein and COP9 expression levels were obviously increased.Conclusion Large dose of hormones induce the disorder of lipid metabolism and differentiation of MSCs,and Folium Cajani enhances the stress reaction and self protection,and promote its osteogenic differentiation through MAPK pathway.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2013年第4期351-355,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金项目(81273784)
关键词
股骨头坏死
骨髓间充质干细胞
激素
蛋白质组学
Necrosis of the femoral head
Bone marrow mesenchymal stem cells
Hormone
Proteomics
作者简介
马春涛，男，博士，研究方向：髋膝关节疾病诊疗。Email：metl983@163．com。
通讯作者：曾意荣，博士，教授，硕士生导师，主任医师，研究方向：髋膝关节疾病的基础与临床研究。Email：zeng6612@163．com
