摘要
目的:评价不同生长期及不同年限葛根多糖含量的动态积累。方法:以葡萄糖为对照品,采用以苯酚-硫酸法,在486 nm处测定不同生长期及不同年限葛根中多糖的含量。结果:在0.020～0.142 mg.L-1葡萄糖质量浓度与吸光度线性关系良好(r=0.999 9),平均回收率99.34%,RSD 1.82%。葛根中多糖的含量呈规律性变化,每年以8,9月份葛根多糖的含量较高,分别为10.28%和11.35%。多年生葛根中多糖含量明显高于前3年,为14.28%。结论:该文结果可为野葛规范化种植及药材采收加工提供科学依据。
Objective： To determine the total polysaccharides in different growth phase and different years of Puerariae lobatae Radix. Method： Total polysaccharides content was also determined by pheno-sulfuric acid method using dextrose as standard sample. Result： Glucose was linear in the range of 0. 020-0. 142 mg （r = 0. 999 9）. The average recovery rate was 99.34% （RSD 1.82% ）. The content of total polysaeeharides appeared regular changes and the content of total polysaccharides was highest in August and September of perennial Puerariae Lobatae Radix. Conclusion ： The result might provide scientific basis for havesting of Puerariae Lobatae Radix.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第16期63-65,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
科技部"十一五"科技支撑计划项目(2006BAI06A10)
关键词
葛根
多糖
动态积累
Puerariae Lobatae Radix
total polysaccharides
accumulative dynamic state
作者简介
纪宝玉，硕士，讲师，从事中药规范化种植及质量标准研究，Tel：13607669844，E—mail：jby19820429@yahoo．com．cn
[通讯作者]陈随清，博士，教授，从事中药品种整理及质量标准研究，Tel：0371-5676686，E—mail：suiqingchen@sohu．com
