摘要
建立了饲料添加剂L-赖氨酸硫酸盐的HPLC测定方法采用氨基酸分析用色谱柱，以流动相A和流动相B进行梯度洗脱，流速为0．45ml／min；采用紫外检测器，检测波长在0～16min为338nm、在16～25min为262nm，结果显示，L-赖氨酸硫酸盐在0～0．25mg／ml范围内，其含量与峰面积呈良好的线性关系，相关系数为0．9996，平均回收率99．0％，RSD为0．8%,本方法准确度高、重现性好，结果准确可靠。
The text established method detecting L-Lysine sulphate in feed additives. With amino acid chromatography colunm, carrying on gradient elution using by mobile phase A and mobile phase B, velocity of flow is 0.45 ml/min;adopted ultraviolet detector, collecting wavelength is 338 nm within 0-16 rain, collecting wavelength is 262 nm within 16-25 rain.The result has indicated thal the content of I,-Lysine sulphate has good linear relation with area in the range of 0-0.25 mg/ml, its correlation coefficient is 0.999 6, its average recovery is 99.0%,RSD is 0.8%. This method is simple and convenient, the reproducibility is excellent, and the results is accurate and safe, the content of L-Lysine sulphate in feed additives can be controlled by this method.
出处
《饲料工业》
北大核心
2013年第14期43-45,共3页
Feed Industry
关键词
L-赖氨酸硫酸盐
氨基酸色谱柱
紫外检测器
HPLC法
L-Lysine sulphate
amino acid chromatography column
ultraviolet detector
high performance liquid chromatography
作者简介
卢琳，硕士，研究方向为兽药检测、乳与乳制品检测、饲料与饲料添加剂的检测及相关方法。
