摘要
目的筛选升麻联合皂角刺(升皂方)抗肿瘤处方,评价其抗肿瘤疗效。方法建立小鼠4T1乳腺癌肺癌转移模型和4T1皮下移植模型,两种模型小鼠均随机分为模型组、升麻组、皂角刺组、升皂方1组、升皂方2组和升皂方3组,每组10只。通过抗肿瘤疗效确定升皂方的比例。MTT法考察升皂方对4T1乳腺癌细胞的抑制作用。用酶联免疫吸附试验(ELISA法)测定荷瘤小鼠血清中Ⅰ型胶原(Col-Ⅰ)、血小板活化因子(PAF)、金属蛋白酶组织抑制剂(TIMP-1)、酪氨酸激酶受体A(TrkA)及酪氨酸激酶受体B(TrkB)的浓度,基质金属明胶酶谱法检测升皂方对基质金属蛋白酶(MMPs)的影响,探索升皂方的抗肿瘤机制。结果与模型组比较,升麻与皂角刺2∶1处方对4T1乳腺癌肺转移作用最明显,肺上的结节数较少,肺转移发生率也较低,且对4T1乳腺癌皮下移植肿瘤的抑瘤率最高。各给药组小鼠血清中的Col-Ⅰ、PAF、TIMP-1、TrkA、TrkB的浓度均降低,以升麻与皂角刺2∶1处方组降低幅度最大,其抑制MMP-2及MMP-9表达的作用最强。结论升麻与皂角刺组方均有不同程度的抗肿瘤作用,当升麻∶皂角刺=2∶1时,抗肿瘤作用最强,为升皂方的最佳配伍。
Objective To screen the anti-tumor prescription（Shengzao Fang） of Shengma（Rhizoma Cimicifugae） combined with Zao Jiao-ci（Spina Gleditsiae）,and evaluate its anti-tumor effects.Methods The lung metastatic model of 4T1 breast cancer and the 4T1 subcutaneous transplanted model were established,both models of mice were randomly divided into model group,Shengma group,Zao Jiao-ci group,Shengzao Fang first group,Shengzao Fang second group and Shengzao Fang third group,10 mice for each group.The proportion of Shengzao Fang was ascertained by their anti-tumor effects.MTT assay was used to investigate the effects of Shengzao Fang on 4T1 breast cancer.The concentration of type 1 collagen（Col-Ⅰ）,platelet-activating factor（PAF）,tissue inhibitor of metalloproteinase 1（TIMP-1）,tyrosine kinase receptor A（TrkA） and tyrosine kinase receptor B（TrkB） in serum from tumor-bearing mice was tested by enzyme-linked immunosorbent assay（ELISA）.The effects of Shengzao Fang on matrix metalloproteinase（MMPs） was examined by Gelatin matrix method.Results Compared with the model group,the prescription of Shengma：Zao Jiao-Ci=2：1 significantly suppressed lung metastasis of 4T1 breast cancer,showing fewer lung nodes,lower lung metastasis rate and highest tumor inhibitory rate to 4T1 subcutaneous transplanted model.Although the concentration of serum Col-Ⅰ,PAF,TIMP-1,TrkA and TrkB was decreased in all treated group,the prescription of Shengma：Zao Jiao-ci=2：1 had the strongest activity,and its inhibitory effect on the expression of MMP-2 and MMP-9 was strongest,too.Conclusion The prescriptions of Shengma combined with Zao Jiao-ci had different antitumor activity,the strongest activity was exhibited when shengma ： Zao Jiao-ci was 2：1,suggesting that 2 ： 1 was optimization for Shengma combined with Zao Jiao-ci.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第8期32-35,共4页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81173094)
河南省科技厅重点攻关项目(112101310308)
河南省高校青年骨干教师项目(2010GGJS-025)
河南大学省部共建项目(SBGJ090704)
关键词
升麻
皂角刺
药物筛选
4T1乳腺癌
小鼠
Keyw words：Shengma
Zao Jiao-ci
drug screening
4T1 breast cancer
mice
作者简介
通讯作者：杜钢军，E-mail：dgj1hh@163．com
