摘要
为了研究小麦穗粒数与小麦雌性育性之间的关系,以含小麦雌性不育系(S)的由小麦品种(系)组成的含S种质双向极端小群体和不含小麦雌性不育系的品种双向极端小群体各60个品种(系)为目标群体,以小麦基因组中42个多态性SSR为背景控制标记,用STRUCTRE软件进行群体结构评估,并利用TASSEL软件的MLM模型检测210个多态性标记与小麦雌性育性I.F.(国际法育性)和D.F.(国内法育性)2种表型值的关联程度,确定入选标记,再对入选标记所在的连锁群进行分析,找到与小麦穗粒数(小穗基部小花数)更加紧密关联的标记。经关联分析,在2个极端小群体里发现,小麦小穗基部小花数关联的标记共有2个(Xgwm570-6A,Xwmc776-4A);2个品种群体中与小麦穗粒数关联的标记共有2个(Xwmc25-2B/2D,Xwmc168-7A)。认为2D、5B连锁群可能有小麦穗粒数形成的遗传位点。
To study the relationship of female fertility and kernel number per spike in wheat,two small populations with 60 varieties or lines alternatively, one called S involved （female sterile line S ）diverse extreme population and another called variety diverse extreme population, were composed as tested populations,42 polymorphic SSR markers in wheat genome were selected as background marker stotest. Its＇ population genetic structure,the MLM mode lins of tware TASSEL was used to check 210 polymorphic markers association degree with phenotype female fertility （international fertility-I.F, and domestic fertility-D.F） ,the highly associated markers with phenotype from small population analysis were verified in relatively big variety population and S involved bigger population,find highly associated markers with kernel number per spike in wheat. Total 2 （Xgwm570-6A ,Xwme776-4A）association markers were found by two small extreme populations with phenotype kernel number per spike. Total 2 （Xwme25-2B/2D ,Xwmc168-7A）assoeiation markers were found by two small variety populations with phenotype kernel number per spike. It was believed that 2D and 5B loci were associatied with kernel number per spike.
出处
《现代农业科技》
2013年第15期9-10,12,共3页
Modern Agricultural Science and Technology
基金
国家自然科学基金项目(30771380
31060189)
关键词
小麦
雌性育性
穗粒数
关联分析
双向极端群体
wheat
female fertility
kernel number per spike
association mapping
diverse extreme population
作者简介
通讯作者
