摘要
[目的]研究高温α-淀粉酶和糖化酶对怀山药的分解效果。[方法]利用高温α-淀粉酶和糖化酶对怀山药分步进行水解糖化以减小分散颗粒直径和沉淀率,在控制一定温度和时间的条件下,采用正交设计和因素分析来确定最适酶解条件,从而提高山药汁的可溶性固形物百分含量,降低其沉淀率和色泽。[结果]试验表明,双酶法分解怀山药的最适酶解条件为:高温α-淀粉酶添加量0.4%(20 000U),山药浓度10%,酶解温度85℃,时间2 h条件下酶解效果最佳;糖化酶添加量0.4%(10 000 U),糖化温度55℃,时间3 h,pH 5.0。[结论]研究可为怀山药的深度开发提供一定的理论依据。
[Objective] To study hydrolyzing effect of high temperature α-amylase and glucoamylase on Huaiqing yam. [Method] High temper- ature a-amylase arid glucoamylase were used to conduct hydrolysis and saccharification, in order to reduce the dispersed particle diameter and sedimentation rate. Under the condition of controlling temperature and time, orthogonal design and factor analysis were adopted to determine the optimal hydrolysis conditions, so as to improve soluble solids content and reduce sedimentation rate and color. [ Result] The optimal hydrolysis conditions are： high temperature α-arnylase 0.4% （20 000 U）, yam 10%, temperature 85℃, time 2 h; Glucoamylase 0.4% （10 000 U）, tem- perature 55 ℃, time 3 h, pH 5.0. [ Conclusion ] The study can provide theoretical basis for deeply development of Huaiqing yam.
出处
《安徽农业科学》
CAS
2013年第14期6462-6465,共4页
Journal of Anhui Agricultural Sciences
关键词
怀山药
高温Α-淀粉酶
糖化酶
可溶性固形物含量
沉淀率
Huaiqing yam
High temperature α-amylase
Glucoamylase
Soluble solids content
Sedimentation rate
作者简介
刘瑞山（1975-），男，河南漯河人，工程师，从事食品工程及应用研究，E-mail：13978889696@163．com。
