摘要
目的:探讨信号转导和转录活化因子1(signal transduction and activators of tran-scription 1,STAT1)基因转染对肺癌移植瘤生长的影响。方法:构建稳定转染STAT1基因(转染组)及空载体(空载体组)的肺腺癌NCL-H1299细胞,使用未转染的细胞及上述两种转染细胞分别建立裸鼠皮下移植瘤模型,连续观察并测量肿瘤生长情况,Western blot法检测肿瘤组织内STAT1活化情况。结果:STAT1基因转染组裸鼠移植瘤接种33 d后瘤体积及瘤重小于未转染组及空载体组(均P<0.05)。Westernblot检测结果提示STAT1转染组的肿瘤组织STAT1表达及磷酸化水平均明显升高。结论:STAT1基因转染可提高移植瘤组织内STAT1活化水平,并对肿瘤生长起抑制作用。
Objective： To investigate the effects of signal transduction and activators of transcription 1 in the progression of lung cancer in vivo. Methods： STAT1 gene or empty vectors were transfected into NCL- H1299 cells with plasmids constructed as previous. Nude mice were transplanted subcutaneously with H1299 cells, H1299 cells transfected with empty vectors, H1299 stably transfected with STAT1, respectively. The size of tumor was examined by caliper every two days. Five weeks after inoculation, the tumors were resected and weighed. Western blot was used to detect the level of STAT1 protein in tumor tissues. Results： Both volume and weight of the tumor in the STATI transfected group were less than those in the other groups （P〈0.05）. Western blot revealed that the protein level of STATI and phosphorylated-STAT1 in STAT1 transfected group were rises notably. Conclusion： The recombinant plasmid STAT1 can significantly suppress the tumor growth of human lung cancer cell NCL-H1299 subcutaneous xenografts in nude mice through up-regulate of STAT1 expression.
出处
《温州医学院学报》
CAS
2013年第7期461-463,共3页
Journal of Wenzhou Medical College
基金
温州市科技局科研基金资助项目(H20080058)
作者简介
马源（1988-），男，安徽芜湖人，硕士生。
通信作者：陈成水，主任医师，教授，硕士生导师，Email：chenchengshui@gmaii．com。
