摘要
目的:建立复方龙脷胶囊中栀子苷和大黄素甲醚的测定方法。方法:采用Waters Symmetry C18色谱柱(4.6mm×250 mm,5μm),流动相分别为乙腈-水(15∶85)、甲醇-水(95∶5),流速1.0 mL.min-1,检测波长分别为238,254 nm,柱温25℃。结果:栀子苷、大黄素甲醚分别在0.493 2～2.959 2μg(r=0.999 5)和0.063 4～0.380 4μg(r=1.000 0)线性关系良好,平均加样回收率分别为100.4%,101.3%,RSD分别为1.9%,1.7%。结论:方法简便可行,结果准确,重复性好,可用于复方龙脷胶囊中栀子苷和大黄素甲醚的含量测定。
Objective： To establish a HPLC method for the determination of Geniposide and Physcion in Compound Longli Capsule.Method： Waters Symmetry C18 analytical column（4.6 mm × 250 mm,5 μm） was used.The mobile phase was consisted of acetonitrile-water（15∶ 85） and methanol-water（95∶ 5） respectively;the flow rate was 1.0 mL.min-1,UV detection wavelength was set at 238,254 nm respectively.The column temperature was maintained at 25 ℃.Result： Under the chromatographic conditions used,the calibration curve of geniposide and physcion was linear in the range of 0.493 2-2.959 2 μg（r = 0.999 5） and 0.063 4-0.380 4 μg（r =1.000 0） respectively.The average recoveries was 100.4% and 101.3% with RSD 1.9% and 1.7% respectively.Conclusion： The method is accurate,effective and feasible.It can be used for the determination of the content of geniposide and physcion contained in Compound Longli capsule.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第14期135-138,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西千亿元重大科技攻关工程项目(桂科攻11107009-2-6)
作者简介
杜成智，讲师，硕士，从事药物分析研究，Tel：0771-2219877，E-mail：276561774@qq．com
[通讯作者]谭建宁，研究生，高级实验师，从事中药化学成分及质量标准研究，Tel：0771-2219877，E-mail：648545814@qq．com
